γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium gishiri Cas: 63699-78-5 Hasken rawaya microcrystalline foda
| Lambar Catalog | XD90183 |
| Sunan samfur | γ-L-Glutamyl-3-carboxy-4-nitroanilide, gishiri monoammonium |
| CAS | 63699-78-5 |
| Tsarin kwayoyin halitta | Saukewa: C12H12N3O7-.H4N+ |
| Nauyin Kwayoyin Halitta | 328.27 |
| Bayanin Ajiya | yanayi |
Ƙayyadaddun samfur
| Bayyanar | Hasken rawaya microcrystalline foda |
| Asay | 99% |
| Wurin narkewa | 187ºC ± 2.0ºC |
| Wurin tafasa | 718.3°C a 760 mmHg |
| Ma'anar walƙiya | 368.5 ° C |
| Solubility | H2O: 100 mg/ml, bayyananne, rawaya-kore |
An bincika kinetics na ɗan adam gamma-glutamyltransferase (EC 2.3.2.2), tare da yin amfani da glycylglycine a matsayin gamma-glutamyl substrate mai karɓa da gamma-glutamyl-4-nitroanilide da kuma abin da ya samo asali na carboxy, gamma-glutamyl-3-carboxy-4 -nitroanilide, a matsayin masu ba da gudummawa.Abubuwan da suka faru na lokaci guda na gamma-glutamyltransfer da autotransfer an kafa su ta hanyar saukowa na chromatography na takarda.Matsakaicin madaidaicin-rabobi biyu-masu-masu-maza suna tabbatar da cewa tsarin enzyme ba shi da mahimmanci (ping-pong bi-bi).Ba a sami hanawa ta kowane mai ba da gudummawa ba, kuma hanawa ta glycylglycine an lura da shi ne kawai a mafi yawan abubuwan da ke da sha'awar asibiti.Matsakaicin kinetic da aka samu ta hanyar bincike na koma baya na bayanan saurin farko an yi amfani da shi don tantance yawan abubuwan da ke haifar da reagent don tantance wannan enzyme.Yin amfani da 4 mmol na gamma-glutamyl-3-carboxy-4-nitroanilide da 100 mmol na glycylglycine a kowace lita ya samar da daidaitattun ayyuka ga waɗanda ta hanyar yin amfani da 4 mmol na gamma-glutamyl-4-nitroanilide da 40 mmol na glycylglycine a kowace lita.Waɗannan ƙididdiga na mai ba da gudummawar carboxy da glycylglycine suma “mafi kyawun farashi” kuma ba su da matsalolin tsari lokacin amfani da su.
