Kayayyaki

1.Optically aiki D-2-hydroxybutanoate ne mai muhimmanci gini block matsakaici ga magunguna da biodegradable poly (2-hydroxybutanoate).Ƙaddamarwar Kinetic na tseren 2-hydroxybutanoate na iya zama madadin kore da kyawawa don samar da D-2-hydroxybutanoate.A cikin wannan aikin, D-2-hydroxybutanoate a babban maida hankali (0.197 M) da kuma babban enantiomeric wuce haddi (99.1%) an samar da wani NAD mai zaman kanta L-lactate dehydrogenase (L-iLDH) dauke da biocatalyst.2-Oxobutanoate, wani muhimmin tsaka-tsaki, an haɗa shi a babban taro (0.193 M).Yin amfani da tsarin musayar ion mai sauƙi tare da macroporous anion resin resin D301, D-2-hydroxybutanoate ya rabu da tsarin biotransformation tare da babban farfadowa na 84.7%.

2.Asymmetric kira na amino acid mara kyau an nuna shi ta omega-transaminase daga Vibrio fluvialis JS17.L-2-Aminobutyric acid an haɗa shi daga 2-oxobutyric acid da benzylamine tare da wuce haddi na enantiomeric fiye da 99%.Halin ya nuna mummunan hana samfurin ta benzaldehyde, wanda aka rinjaye shi ta hanyar yin amfani da tsarin amsawa na biphasic don cire samfurin hanawa daga yanayin ruwa.A cikin halayen biphasic na al'ada (50 mM 2-oxobutyric acid, 70 mM benzylamine da 2.64 U/ml purified enzyme) ta yin amfani da hexane a matsayin mai cirewa, tuba na 2-oxobutyric acid ya kai 96% a cikin 5 h yayin da kawai 39% tuba aka samu ba tare da fitar da samfurin.