3- [(3-Cholanidopropyl) dimethylamonio] -1 -propanesulfonate Cas: 75621-03-3 98%
Lambar Catalog | XD90075 |
Sunan samfur | 3-[(3-Cholanidopropyl) dimethylammonio]-1-propanesulfonate. |
CAS | 75621-03-3 |
Tsarin kwayoyin halitta | Saukewa: C32H58N2O7S |
Nauyin Kwayoyin Halitta | 614.88 |
Bayanin Ajiya | yanayi |
Jituwa Code Tariff | 29242970 |
Ƙayyadaddun samfur
IR | Kwatanta da tunani |
Asara akan bushewa | <2% |
TLC | Tabo daya |
Nitrogen | 4.46 - 4.65 |
Bayyanar | Farin foda |
Solubility (10% a cikin H2O) | bayyananne kuma mara launi |
Ayyukan aiki (0.1M a cikin H2O) | <10us/cm |
Farashin HPLC | >98% |
UV (5% a cikin H2O) A290nm | <0.5 |
UV (5% a cikin H2O) A400nm | <0.02 |
Abubuwan da ke narkewa (ta GC) Dimethylformamide | <2.0% |
3-[3- (cholamidopropyl) dimethylamino] propanesulfonic acid gishiri na ciki amfani da hanyar kira
Ayyukan Halittu: CHAPS, wanda aka samu daga Cholicacid, wani abu ne na zwitterionic wanda ke narkar da sunadaran membrane.Ana amfani da CHAPS don daidaita rukunin furotin-DNA daban-daban kuma yana iya riƙe ayyukan sinadarai na sunadaran a cikin bayani.
Yana amfani da: CHAPS shine wanki na zwitterionic mara cirewa don kwayoyin halitta na membrane.Ana iya amfani da su don narkewa sunadaran membrane da rushe hulɗar furotin-gina jiki.CHAPS yana da ƙananan nauyin ƙwayoyin micellar (6,150) da babban taro mai mahimmanci (6-10 mM) kuma ana iya cire shi daga samfurin ta hanyar dialysis.Hakanan ya dace da solubilizing sunadaran a cikin mayar da hankali kan isoelectric da 2D electrophoresis.Ana amfani da CHAPS akai-akai a cikin mayar da hankali na isoelectric na asali (ba tare da urea ba) kuma an gano yana ba da kyakkyawan ƙuduri don wasu shirye-shiryen ƙananan ƙwayoyin cuta da sunadaran shuka.Yawanci ƙididdigewa tsakanin 2-4% (w/v) ana amfani da su don isoelectric mayar da hankali gels.
Yana amfani da: Wancan wanki na zwitterionic wanda ba ya denaturing don membrane biochemistry, wanda za a iya amfani da shi don narkewa sunadaran membrane kuma ya rushe hulɗar furotin-protein;kuma dace da solubilizing sunadaran a isoelectric mayar da hankali da biyu-girma electrophoresis;wanda aka saba amfani dashi a cikin Ba-denaturing (ba urea) isoelectric mayar da hankali;RNA da hakar DNA, babu aikin RNase da DNA
Yana amfani da: Amphoteric surfactant, CHAPS wani wanki ne na zwitterionic wanda ba ya denaturing, furotin lysate don solubilizing sunadaran membrane da ma'amalar karya tsakanin sunadarai da sunadarai.