Kayayyaki

Chemifluorescence: Molecular luminol wani sinadari ne mai kyalli wanda za'a iya jujjuya shi zuwa aminophthalic acid mai farin ciki a gaban kwayoyin hydrogen peroxide, wanda ke fitar da haske mai ƙarfi.Hydrogen peroxide shine samfurin halayen halayen biooxidative da yawa, don haka yana da sauƙi a haɗa waɗannan halayen biooxidative tare da gano hoto ta hanyar gabatar da luminol.Misali, binciken glucose oxidase / catalase na iya gano yawan adadin hydrogen peroxide ko glucose a cikin samfurin, kuma lokacin amsawa shine kawai 0.5s (hanyar tsauri).Haɗa abubuwan chemiluminescent tare da halayen rigakafi, da bayyana yawan abubuwan da aka gano na rigakafi ta hanyar halayen haske, ana kiransa fasahar immunoassay na chemiluminescent.A cikin 1976, Shmeder ya ba da shawarar chemiluminescence immunoassay, ta amfani da luminol-H202 da wanda ya samo asali ABEI azaman tsarin nunin amsawa.A halin yanzu, luminol da abubuwan da suka samo asali sune alamomin da aka fi amfani da su a cikin aikace-aikacen immunoassay na chemiluminescence.A ƙarƙashin yanayin alkaline, wanda microperoxidase ya daidaita, ana iya fitar da adadi mai yawa na photon.Ana iya amfani da Luminol don WesternBlot na HRP-labeled antibodies da nucleic acid hybridization ganewa na HRP-labeled bincike.Ana kuma amfani da ita wajen gano tabo jini na zamani.Wani fili wanda za'a iya amfani dashi azaman alamar chemiluminescent dole ne ya hadu da waɗannan sharuɗɗan: yawan yawan adadin haske yana da girma;Properties na physicochemical sun dace da tsarin da ake nazari;halayensa na luminescent shine sakamakon halayen iskar shaka na luminescent;a cikin kewayon maida hankali da aka yi amfani da su na ciki marasa guba ga rayayyun halittu.Yawancin nau'ikan reagents na chemiluminescence da yawa ana amfani da su: acridine ester, mai binciken chemiluminescence da ake amfani da shi sosai, wani fili ne na tricyclic Organic, mai sauƙin oxidize, kuma yanayin iskar shaka baya buƙatar mai haɓakawa, kuma yana fitar da photons a 430nm;Luminol da isoluminol da abubuwan da suka samo asali sune manyan jami'ai na chemiluminescent.A farkon 1964, Yayin da aka ba da rahoto game da chemiluminescence, luminol, isoluminol da abubuwan da suka samo asali an yi amfani da su don chemiluminescence.Immunoassays sun sami sakamako mafi kyau.Ganewar Luminescence: mafi kyawun tsayin kyalli mai haske shine 400nm (gano chemiluminescence a cikin 60mmK2S2O8, 100mmK2CO3, pH11.5 bayani)

Chemiluminescence: A matsayin hanyar tantancewa mai mahimmanci, ana amfani da chemiluminescence sosai don gano radicals kyauta da halayen halayen da aka samar a cikin enzymes, sel da kwayoyin halitta.Ana iya amfani da hasken da samfuran oxidation ɗin su da metabolites ke fitarwa tare da na'urori daban-daban.ganowa.Ana amfani da Chemiluminescence sosai a cikin dubawa da bincike na antioxidants, irin su polyphenols, polysaccharides, flavonoids, da anthraquinones, saboda hankalinsa, saurin sauri, aiki mai sauƙi da ƙarancin farashi.Tsarin chemiluminescence da aka saba amfani da shi don tantance anions superoxide sun haɗa da xanthine oxidase-luminol, pyrogallol-luminol da dimethyl sulfoxide-luminol.Na farko shi ne tsarin enzymatic, na biyun kuma tsarin da ba na enzymatic ne.Tsarin chemiluminescence don ƙaddarar radicals na hydroxyl galibi sun haɗa da jan karfe sulfate-yiast (ko sel marrow) -ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate buffer, jan karfe sulfate-o-phenanthroline-ascorbic acid-hydrogen peroxide 5 chemiluminescence tsarin, ferrous sulfate-luminol-hydrogen peroxide da ferrous sulfate-luminol, duk sun unsa da classic Fenton dauki don samar da hydroxyl radicals, sa'an nan kai farmaki da luminescent wakili don samar da chemiluminescence, wanda za a iya amfani da su auna kau da tsantsa. aiki na reactive oxygen radicals.Hanyar gwaji: amfani da pyrogallol-luminol chemiluminescence tsarin: luminol an yi shi a cikin 0.05mol / L maida hankali bayani tare da 0.05mol / L NaOH bayani, adana a cikin duhu wuri, da distilled ruwa sau biyu kafin amfani da Dilute zuwa 1mmol/L bayani, yi amfani da 1mmol/ L HCl don yin 0.01mol/L bayani na pyrogallol, adana shi a cikin firiji a 4 ° C, kuma a tsoma shi da ruwa mai ninki biyu zuwa sau 16 6.25 × 10-4mol / L bayani kafin amfani.0.05mol/L pH10.2Na2CO3-NaHCO3 buffer (dauke da 0.1mmol/LEDTA) da aka shirya kafin amfani, da kuma gauraye da 1mmol/L luminol a 2: 1 (juzu'in juzu'i) don samar da luminol da carbonate buffer kafin gwajin cakuda.A lokacin ma'auni, allurar 10.0 μL samfurori na ƙididdiga daban-daban (0, 0.08, 0.4, 2 da 10 mg / ml) a cikin tantanin halitta mai haske (tare da buffer samfurin azaman sarrafawa), sannan allurar 6.25 × 10-4mol / L pyrogallol. 0.05 A ƙarshe, an ƙara 0.94 ml na cakuda luminol da buffer carbonic acid don fara amsawa (30 ° C), an ƙididdige ƙarfin haske a tsaka-tsakin 2 s, kuma an ƙididdige yawan ƙarfin luminescence mai ƙarfi a 300 s.Ƙarfin haske na baya shine hasken haske lokacin da ba a ƙara pyrogallol ba.darajar.Bugu da ƙari, akwai chemiluminescence, fluorescence spectroscopy da kuma tsarin NBS-dichlorofluorescein chemiluminescence tsarin don ƙayyade ƙwanƙwasa ƙwanƙwasa, da dai sauransu, babban fa'idar su shine babban hankali.

Chemical Properties: Yellow crystalline foda.Sauƙi mai narkewa a cikin lemun tsami, mai narkewa a cikin acid dilute, kusan ba zai iya narkewa cikin ruwa, kuma ba a narkewa a cikin barasa.Maganin tsaka-tsaki ko dan kadan na acidic suna nuna haske mai haske mai haske lokacin da aka fallasa shi zuwa hasken ultraviolet.Matsayin narkewa 329-332 ℃

Amfani: A matsayin reagen bincike na sinadarai da mai nuna alama.Yi amfani da reagent bincike na Chemiluminescence (kamar ƙayyadaddun cations na ƙarfe ko jini)

Yana amfani da: Don nazarin chemiluminescence, kamar: cations karfe, jini da glucocorticoids

Yana amfani da: Gwajin Luminescence: Emmax440nm (chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; bayan ƙara H2O2) chemiluminescence reagent da nuna alama, wanda aka saba amfani dashi a cikin binciken chemiluminescence, kamar cations na ƙarfe, rigakafi na jini, da sauransu.