Kayayyaki

1.Mun yi nazarin tasirin hanawa na isorhamnetin akan tyrosinase naman kaza ta hanyar hana motsin motsi da kuma lissafin lissafi.Isorhamnetin ya sake hana tyrosinase a cikin nau'in nau'i mai nau'i a Ki = 0.235 ± 0.013 mM.Ma'auni na intrinsic da 1-anilinonaphthalene-8-sulfonate (ANS) -daure fluorescence ya nuna cewa isorhamnetin bai haifar da gagarumin canje-canje a cikin tsarin sakandare na tyrosinase ba.Don samun haske game da tsarin rashin kunnawa, an ƙididdige motsin motsi ta hanyar ma'aunin tazara na lokaci da ci gaba da halayen substrate.Sakamakon ya nuna cewa rashin kunnawa da isorhamnetin ya haifar shine tsari na farko tare da matakan biphasic.Don samun ƙarin haske, mun gwada docking tsakanin tyrosinase da isorhamnetin.Kwaikwayo ya yi nasara (dauri makamashi don Dock6.3: -32.58 kcal / mol, don AutoDock4.2: -5.66 kcal / mol, da kuma Fred2.2: -48.86 kcal / mol), yana nuna cewa isorhamnetin yana hulɗa tare da sauran ragowar, irin wannan. kamar HIS244 da MET280.Wannan dabarar hulɗar tyrosinase ta tsinkaya a hade tare da motsin motsa jiki dangane da wani fili na flavanone na iya tabbatar da amfani wajen tantance masu hana tyrosinase na halitta.

2.Acid bayyana hanyar conalbumin (CA), monomeric glycoprotein daga hen kwai fari, an bincika ta amfani da nisa- da kusa-UV CD spectroscopy, intrinsic fluorescence emission, extrinsic fluorescence bincike 1-anilino-8-napthalene sulfate (ANS) da kuma rarraba haske mai ƙarfi (DLS).Muna lura da canje-canje masu dogaro da pH a cikin tsarin sakandare da na uku na CA.Yana da tsarin sakandare na α-helical na asali a pH 4.0 amma tsarin asarar a pH 3.0.CA ta wanzu na musamman azaman jihar globule da aka riga aka narkar da ita da narkakken yanayin globule a cikin bayani a pH 4.0 da pH 3.0, bi da bi.Tasirin pH akan daidaituwa da ma'aunin zafi na CA yana nuna juriya na zafi a tsaka tsaki pH.Sakamakon DLS ya nuna cewa jihar MG ta kasance azaman ƙaramin tsari a cikin mafita mai ruwa da ruwa tare da radiyo na hydrodynamic na 4.7 nm.Quenching na tryptophan fluorescence ta acrylamide ya kara tabbatar da tarin jaha mai tsaka-tsaki, wani bangare da ba a bayyana ba, tsakanin jahohi na asali da na asali.