Kayayyaki

1.Mun bincika da kuma kwatanta sakamakon biyar H1-antihistamines a kan tsokane oxidative fashe a extra- da kuma intracellular matakin ware da kuma motsa mutum polymorphonuclear leukocytes. Matsayin matsayi na ƙarfin don H1-antihistamines don rage chemiluminescence an kimanta shi extracellularly: dithiaden> loratadine> chlorpheniramine> brompheniramine> pheniramine da kuma a intracellular site: loratadine> dithiaden.H1-antihistamines bambanta sosai bisa ga tsarin sinadaran su a cikin abubuwan da suka fi dacewa da su a cikin abubuwan da ke haifar da su. - da kuma wurin cikin salula na keɓaɓɓen neutrophils na ɗan adam.

2.Chemiluminescence tsarin inganta ta ko dai isoluminol ko luminol a hade tare da peroxidase ne m hanyoyin don gano reactive oxygen jinsunan (ROS) samar da phagocyte NADPH oxidase.Abubuwan haɓakawa guda biyu suna kama da tsari sosai, sun bambanta kawai a matsayin rukunin amino a cikin zoben kamshi na kwayoyin.Wannan bambance-bambancen yana sanya isoluminol ya zama ƙarancin ƙwayar lipophilic wanda ba shi da ƙarfi ga membranes na halitta.Yin amfani da isoluminol saboda haka an iyakance shi ga nazarin da ke da alaƙa da ɓoyewar metabolites na oxygen.A cikin wannan binciken mun nuna cewa peptides na roba da aka samo daga yankin N-terminal na calcium-regulated protein annexin AI yana tsoma baki tare da gano radicals a cikin isoluminol-amplified, amma ba a cikin luminol-amplified, tsarin.Annexin AI-samu peptides rage haske fitarwa tare da isoluminol m da superoxide da horseradish peroxidase (HRP) a cikin formyl-methionyl-leucyl-phenylalanine- da phorbol my ristate acetate-stimulated Kwayoyin, kazalika da hydrogen peroxide da HRP.Ba a san ainihin hanyar hanawa ba.Sakamakon da aka gabatar da karfi yana ba da shawarar cewa rage martanin salon salula da aka gano tare da haɓakar sinadarai na isoluminol ya kamata a tabbatar da shi tare da wata hanya ta dabam don tantance sakin anions na superoxide da hydrogen peroxide.