Kayayyaki

Ƙarfin Clostridium thermocellum don lalata cellulose da sauri da haɓaka sakamakon samfuran hydrolysis zuwa ethanol ya sa ya zama ƙirar dandamali mai ban sha'awa don samar da biofuel na cellulosic ta hanyar haɗin gwiwar bioprocessing.A halin yanzu, duk da haka, yawan amfanin ƙasa na ethanol ya yi ƙasa da matsakaicin ƙayyadaddun ƙa'idodi saboda rassan hanyoyin samfur waɗanda ke karkatar da carbon da electrons zuwa formate, H2, lactate, acetate, da amino acid ɗin da aka ɓoye.Don karkatar da motsin carbon da electron daga tsarin, an goge kwayoyin halittar pyruvate:formate lyase (pflB) da kuma PFL-activating enzyme (pflA).An kawar da samar da tsari a cikin sakamakon Δpfl kuma samar da acetate ya ragu da 50% akan matsakaici da matsakaici.Yawan girma na nau'in Δpfl ya ragu da ninki 2.9 akan matsakaicin matsakaici da haɓakar biphasic akan matsakaicin matsakaici.Ƙarin ƙayyadaddun matsakaici tare da tsarin 2 mM maido da ƙimar girma na Δpfl zuwa 80% na nau'in iyaye.An tattauna rawar pfl a cikin dabarun injiniya na rayuwa da C1 metabolism.