Kayayyaki

Neuroblastoma, ciwon daji na tsarin juyayi mai tausayi, shine mafi yawan ƙwayar ƙwayar ƙwayar cuta a cikin yara.Ƙarawa na MYCN da ƙara yawan siginar BDNF/TrkB sune siffofi na ciwace-ciwacen haɗari;duk da haka, kawai kashi 25% na ciwace-ciwacen ciwace-ciwace suna nuna waɗannan fasalulluka.Don haka, gano ƙarin alamun halittu da makasudin warkewa yana da mahimmanci.Kamar yadda aminoacylase 1 (ACY1), amino acid deacetylase, shine mai hana kumburin ƙwayar cuta a cikin ƙananan ƙwayoyin huhu da carcinomas na koda, mun bincika ko shi ko sauran dangin aspartoacylase (ASPA, aminoacylase 2) ko aminoacylase 3 (ACY3) zasu iya yin hidima. irin wannan aiki a cikin neuroblastoma.An yi nazarin maganganun Aminoacylase a cikin TrkB-positive, MYCN-amplified (SMS-KCNR da SK-N-BE) da TrkB-negative, wadanda ba MYCN-amplified (SK-N-AS, SK-N-SH, SH-SY5Y da kuma SH-EP) neuroblastoma cell Lines.Kowane aminoacylase ya baje kolin keɓancewar wuri (watau cytosolic ACY1, ASPA mai alaƙa da membrane da ACY3).Lokacin da aka bi da ƙwayoyin SK-N-SH tare da wakilai na bambance-bambancen jijiyoyi (misali, retinoic acid da cAMP) a cikin kafofin watsa labaru masu ɗauke da 10% serum, ACY1 shine kawai aminoacylase wanda maganganunsa ya inganta.An bayyana ASPA da farko a cikin sel SH-EP na glial sublineage.ACY3 an fi bayyana shi sosai a cikin TrkB-tabbatacce, MYCN-layukan haɓaka.Dukkanin aminoacylase guda uku an bayyana su a cikin glandar adrenal na ɗan adam na yau da kullun, wurin gama gari na asalin neuroblastoma, amma ACY1 da ACY3 kawai sun nuna maganganun da za a iya ganowa a cikin ƙwayar cutar neuroblastoma ta farko.Ma'adinin bayanan bioinformatics na rayuwa na Kaplan-Meier ya bayyana cewa babban ACY3 magana yana da alaƙa da rashin hangen nesa, yayin da ƙarancin magana na ACY1 ko ASPA yana da alaƙa da rashin fahimta.Wadannan bayanan sun nuna cewa maganan aminoacylase yana dysregulated a cikin neuroblastoma