Kayayyaki

Sabbin na'urori na Trinder sune abubuwan da aka samo asali na aniline masu narkewa da ruwa waɗanda ake amfani da su sosai a cikin gwaje-gwajen bincike da gwaje-gwajen sinadarai.Akwai fa'idodi da yawa akan reagents na chromogenic na al'ada a cikin ƙayyadaddun launi na ayyukan hydrogen peroxide.Sabbin na'urorin na Trinder sun tsaya tsayin daka don amfani da su duka biyun mafita da tsarin gano bututun gwaji.A gaban hydrogen peroxide da peroxidase, an nuna novel Trinder's reagent don amsawa tare da 4-aminoantipyrine (4-AA) ko 3- methylbenzothiazole sulfonehydrazone (MBTH) yayin halayen haɗin haɗin gwiwa.Forms sosai barga violet ko shudi dyes.Ƙunƙarar ƙwanƙwasa na rini tare da MBTH ya kasance 1.5- 2 sau fiye da na rini tare da 4-AA;duk da haka, maganin 4-AA ya fi kwanciyar hankali fiye da maganin MBTH.The substrate ne enzymatically oxidized ta oxidase don samar da hydrogen peroxide.Matsakaicin hydrogen peroxide yayi daidai da ƙaddamarwar substrate.Sabili da haka, ana iya ƙayyade adadin adadin ta hanyar haɓakar launi na haɓakar haɗin gwiwar oxidative.Glucose, barasa, acyl-CoA da cholesterol za a iya amfani da su don gano waɗancan abubuwan da aka haɗa zuwa littafin Trinder's reagent da 4-AA.Akwai sabbin reagents 10 na Trinder.Daga cikin sabbin kayan aikin Trinder, TOOS shine mafi yawan amfani.Koyaya, don takamaiman ƙayyadaddun kayan aiki, gwada azuzuwan daban-daban na reagents na Trinder yana da mahimmanci don haɓaka ingantaccen tsarin ganowa.