Kayayyaki

Ayyukan Halittu: BCIP (BCIP p-toluidine gishiri; X-phosphate p-toluidine gishiri) wani abu ne na chromogenic don gano launi na aikin alkaline phosphatase.

Shiga gubobi (Stxs) ana samar da shi ta hanyar enterohemorrhagic Escherichia coli (EHEC), wanda ke haifar da cututtukan ɗan adam tare da sakamako mai saurin mutuwa.Layukan salula na Vero, waɗanda aka samo daga koren biri na Afirka, suna wakiltar ma'aunin zinariya don tantance tasirin cytotoxic na Stxs.Duk da amfani da su na duniya, sani game da ainihin tsarin Stx glycosphingolipids receptor glycosphingolipids (GSLs) da taron su a cikin rafts na lipid ba shi da kyau.Anan mun gabatar da cikakken tsarin bincike na Stx receptor GSLs da rarraba su zuwa membranes masu jure wa wanka (DRMs), waɗanda aka shirya daga ƙwayoyin Vero-B4 kuma ana amfani da su azaman daidaitaccen rafin lipid.Mun gano globotriaosylceramide (Gb3Cer) da globotetraosylceramide (Gb4Cer) a matsayin masu karɓar GSL don ƙananan nau'ikan Stx1a, Stx2a, da Stx2e ta amfani da gano mai rufin TLC hade da MS.Mai karɓa na Stx wanda ba a sani ba, globopentaosylceramide (Gb5Cer, Galβ3GalNAcβ3Galα4Galβ4Glcβ1Cer), wanda aka gane musamman (ban da Gb3Cer da Gb4Cer) ta Stx2e, an siffanta shi sosai.Lipoforms na Stx receptor GSLs an samo su da yawa suna ɗaukar nau'ikan ceramide waɗanda suka ƙunshi sphingosine (d18:1) da C24: 0/C24: 1 ko C16: 0 fatty acid.Bugu da ƙari, haɗuwa tare da alamomin raft, SM da cholesterol, a cikin DRMs sun ba da shawarar haɗin GSL tare da microdomains na membrane.Wannan binciken yana ba da tushe don ƙarin bincika tasirin aikin masu karɓa na Stx masu haɗin lipid raft don raunin da ya haifar da toxin na ƙwayoyin Vero-B4.