Kayayyaki

Bicine shine buffer amino acid zwitterionic, mai aiki a cikin kewayon pH 7.6-9.0 (pKa na 8.26 a 25°C).Shawarwarin buffer don ƙarancin zafin aikin biochemical.Ana amfani da Bicine don shirye-shiryen tsayayyen bayani don tantance maganin guanase.An buga amfani da bicine a cikin siraren ion musayar hanyar chromatography don ƙudurin furotin.An yi amfani da Bicine a cikin peptide da crystallization na furotin.Nazarin motsin rai na ƙayyadaddun yanayin canji-jihar analogue na creatine kinase da aka yi amfani da bicine a cikin ma'ajin amsawa.An kwatanta tsarin buffer multiphasic don SDS-PAGE na sunadaran da peptides waɗanda suka haɗa da bicine.

Ganuwar alveolar guda ɗaya da aka ƙaddamar da nazarin tsayi-tsayi a cikin saline da Bicine (0.2 M) suna ci gaba da lalacewa a cikin tashin hankali na nama (TTD).Mun bincika tasirin mafita daban-daban akan wannan TTD kuma mun nemi sauye-sauye masu dacewa a cikin ultrastructure.An rarraba parenchyma na huhu zuwa bangon alveolar guda ɗaya (30 X 30 X 150 microns) a cikin salin phosphate-buffered (0.15 M).Canja wurin zuwa wanka mai tsayi mai tsayi, an nutsar da nama a cikin Bicine, Saline, Maganin Hank mai ƙarfi, 0.25% Alcian blue a cikin saline, ko maganin sodium dodecyl sulfate, don lokuta masu canzawa.An zagaya ta hanyar tsawaita da aka auna tare da mafi girman ƙarfin da aka auna akan lokaci, waɗannan kyallen takarda iri ɗaya an gyara su a cikin buffered glutaraldehyde/tannic acid kuma ana sarrafa su don microscopy na lantarki.Ganuwar alveolar guda ɗaya da aka nutsar a cikin saline ko Bicine sun nuna TTD mai ci gaba.Vacuoles ko sarari sun bayyana a cikin interstitium wanda tare da rashin tsari na salula ya ci gaba tare da TTD.An gani a cikin sa'o'i 0.3, canje-canjen sun ci gaba sosai a cikin sa'o'i 0.6.A cikin sodium dodecyl sulfate (70 mM), duk da haka, babu TTD kuma a tsarin babu wani interstitium, tare da membranes na ginshiƙai kawai da kuma sunadaran fibrous.A cikin ingartaccen maganin Hank ko 0.25% Alcian blue an adana matrix interstitial, ilimin halittar tantanin halitta da tashin hankali na nama na sa'o'i 1 da kyau.Wannan binciken ya nuna cewa leaching na interstitial matrix yana faruwa a cikin saline ko Bicine, kuma matrix mai mahimmanci yana da mahimmanci don adana tashin hankali na nama.