Kayayyaki

Buffer, ana amfani dashi don gano creatine-creatinase;kuma ana amfani da shi don ingantaccen kariya na haemoglobin a bushe-bushe;catholyte, ana amfani dashi a cikin IEF/2-D gel electrophoresis.

Glial cell line-derived neurotrophic factor (GDNF) shine mai hanawa mai amfani da ethanol da sake dawowa, kuma GDNF heterozygous knockout mice yana nuna ƙara yawan lada ga ethanol kuma yana cinye ƙarin ethanol bayan wani lokaci na abstinence fiye da abokan cinikin su na daji.Anan, mun gwada ko ethanol ya canza GDNF magana a cikin yanki na ventral tegmental (VTA; GDNF's site na aikin) da / ko tsakiya accumbens (NAc; babban tushen GDNF), kuma idan haka ne, ƙayyade rawar da haɓakar haɓakar haɓakawa. a cikin tsari na amfani da ethanol.Gudanar da tsarin ethanol ya karu GDNF magana da matakan furotin a cikin VTA, amma ba NAc ba.Bugu da ƙari, an haɓaka matakan GDNF bayan zaman shan ethanol a cikin berayen da suka cinye ethanol a cikin tsaka-tsakin hanyar zaɓin kwalba biyu na sati 1, amma ba makonni 7 ba.Ragewa bayan makonni 7 na yawan amfani da ethanol ya rage matakan GDNF, yayin da ɗan gajeren lokaci na ethanol binge sha bayan rashi ya inganta GDNF magana.Mahimmanci, ƙwanƙwasa GDNF a cikin VTA ta amfani da adenovirus yana bayyana gajeriyar gashin gashi RNA ya sauƙaƙe haɓakar shan ethanol ta berayen-naïve, amma ba ta berayen da ke da tarihin yawan amfani da ethanol ba.Wadannan sakamakon sun nuna cewa a lokacin abubuwan sha na farko na ethanol, GDNF a cikin VTA ya karu kuma yana kare kariya daga ci gaba da yawan cin abinci na ethanol.Koyaya, martanin kariyar haɓakar haɓaka ga ethanol ya rushe bayan tsawan lokacin shan ethanol da kuma janyewa, yana haifar da nacewa, yawan amfani da ethanol.