Kayayyaki

Sabbin acid butanesulfonic na zwitterionic wanda ke fadada kewayon alkaline na iyalai hudu na kyawawan buffers: kimantawa don amfani a cikin tsarin ilimin halitta.

Sabbin buffers guda huɗu na zwitterionic butanesulfonic acid waɗanda ke da alaƙa da tsari ga iyalai huɗu na Kyakkyawan buffers an kimanta su don amfani da su a cikin tsarin nazarin halittu.Wadannan buffers, tare da ƙimar pKa daga 7.6 zuwa 10.7, an kwatanta su tare da wasu nau'o'in nau'i na daban-daban daga iyali guda kuma tare da ma'auni marasa alaƙa don ƙayyade tasirin su akan ayyukan enzyme da kuma ci gaban ƙananan ƙwayoyin cuta.Ayyukan enzymes hudu tare da mafi kyawun ƙimar pH a cikin kewayon alkaline an gwada su: beta-galactosidase, esterase, phosphodiesterase da alkaline phosphatase.Gabaɗaya, duk kyawawan buffers, gami da sabbin buffers na butanesulfonic acid, sun ba da kyakkyawan aiki;duk da haka, akwai bambance-bambance a cikin ayyukan wasu enzymes tare da wasu buffers.Tris, glycine, da phosphate buffers yawanci suna nuna bambancin ayyuka idan aka kwatanta da dangin Good buffers.beta-Galactosidase, musamman, ya nuna babban aiki tare da kyawawan buffers fiye da phosphate ko Tris buffers.Hakazalika, haɓaka nau'ikan ƙwayoyin cuta guda bakwai ya yi daidai, tare da ƴan keɓantawa, ga duk Kyawawan dangin masu buffer tare da Tris galibi suna hana girma.Ƙididdigar phosphatase na alkaline da aka haɗa zuwa ƙwayoyin rigakafi shine kayan aiki mai mahimmanci a yawancin aikace-aikace a cikin ilmin kwayoyin halitta.Yawancin kyawawan buffers sun ba da sigina masu kyau idan aka kwatanta da Tris a pH 9.5 don gano sunadaran ta amfani da ƙwayoyin rigakafi masu haɗaka da alkaline phosphatase.