Kayayyaki

An yi amfani da shi don ƙirƙirar buffer CAPS, buffer zwitterionic mai amfani a cikin kewayon pH 7.9-11.1.Ana amfani da buffer na CAPS sosai a cikin gwaje-gwajen Yamma da na rigakafi da kuma jerin abubuwan gina jiki da ganowa.An yi amfani dashi a cikin hanyar canja wurin sunadarai zuwa PVDF (sc-3723) ko nitrocellulose membranes (sc-3718, sc-3724).Babban pH na wannan buffer yana sa ya zama mai amfani don canja wurin sunadarai tare da pI> 8.5.da ƙarancin amsawa tare da enzymes ko sunadarai, ƙarancin tasirin gishiri.

A cikin yanki na capillary electrophoresis, saurin electrophoretic na ion yana raguwa yayin da aka ƙara tattara bayanan bayanan electrolyte.Wannan yana faruwa ne ta hanyar canje-canje a cikin motsi na electrophoretic na ion (muep) da kuma canje-canje a cikin ƙarfin net ɗin da ke shafar shi, wato ƙarfin filin lantarki mai tasiri (Eeff).Motsi na electrophoretic na ion yana canzawa ta canje-canje a cikin cikakken danko na maganin electrolyte da canje-canje a cikin narkar da girman ion.Eeff yana canzawa musamman ta canje-canje a girman tasirin cajin asymmetry da tasirin electrophoretic, duka biyun suna jinkirta motsin ions.A cikin wannan binciken, an yi amfani da fasaha mai alamar uku don nazarin tasirin tasirin electrolyte na baya (0.02-0.08M 3-[cyclohexylamino] -1-propanesulfonic acid da counter ion (Li, Na, K, da Cs) akan Eeff. An gano cewa maida hankali na electrolyte na baya yana tasiri sosai ga Eeff kuma Eeff yana fuskantar E yayin da maida hankali na electrolyte na baya ya kusanci sifili. , Eeff ya ragu. Dabarar mai alamar uku ta tabbatar da dacewa don irin wannan ƙaddara.