Kayayyaki

brucellae sune magungunan etiological na brucellosis, zoonosis da aka rarraba a duniya.Waɗannan ƙwayoyin cuta ne na ƙwayoyin cuta na cikin salula kuma don haka suna iya daidaita metabolism ɗin su zuwa yanayin karin- da na cikin salula da aka fuskanta yayin zagayowar kamuwa da cuta.Koyaya, wannan bangare na ilimin halitta na Brucella ba a fahimta sosai ba, kuma ba a san abubuwan gina jiki da ke cikin alkukin salula ba.Anan, mun bincika tsakiyar hanyoyin C metabolism da Brucella abortus yayi amfani da shi ta hanyar share fructose-1,6-bisphosphatase (fbp da glpX), phosphoenolpyruvate carboxykinase (pckA), pyruvate phosphate dikinase (ppdK), da malic enzyme (mae) kwayoyin halitta.A cikin gluconeogenic amma ba a cikin kafofin watsa labaru masu wadata ba, haɓakar ΔppdK da Δmae mutants sun lalace sosai kuma an rage girman nau'in Δfbp-ΔglpX sau biyu.A cikin macrophages, kawai ΔppdK da Δmae mutants sun nuna raguwar haɓakawa, kuma nazarin tare da ΔppdK mutant ya tabbatar da cewa ya kai ga abin da aka yi.Hakazalika, kawai ΔppdK da Δmae mutants an rage su a cikin mice, tsohon ana share shi ta mako 10 kuma na karshen ya ci gaba fiye da makonni 12.Mun kuma bincika sake zagayowar glyoxylate.Kodayake aceA (isocitrate lyase) mai haɓaka ayyukan haɓakawa ya haɓaka a cikin matsakaicin matsakaici, rushewar aceA ba ta da tasiri a cikin vitro ko kan ninkawa a cikin macrophages ko ƙwayoyin linzamin kwamfuta.Sakamakon ya nuna cewa B. abortus yana tsiro a cikin salula ta hanyar amfani da iyakataccen wadataccen abinci na 6-C (da 5-C) wanda aka biya ta glutamate da yiwuwar sauran amino acid da ke shiga cikin zagaye na Krebs ba tare da muhimmiyar rawa na glyoxylate shunt ba.