Kayayyaki

Spiral tube taro counter-chromatography na yanzu an yi nasarar amfani da shi a cikin haɓakar dl-tryptophan ta amfani da ƙwayar ƙwayar ƙwayar cuta ta bovine a matsayin mai zaɓin chiral.Ingantacciyar tsarin narkewa mai ruwa-ruwa mai ruwa-ruwa 12.0% (w / w) polyethyleneglycol 8000-9.0% (w / w) dibasic potassium phosphate-0.1% ammonia-78.9% ruwa an yi amfani dashi azaman tsarin narkewa don chromatography na yanzu. Serum albumin na bovine an fi rarraba shi a cikin ƙananan lokaci na tsarin ruwa mai kashi biyu.Tsarin kaushi mai ruwa-ruwa ya ba da babban ƙarfin kuzari don d- da l-tryptophan a α=2.605 tare da rabon rarraba DD=1.200 da DL=0.461.An sami babban ƙuduri mai girma don haɓaka 2.0mg na dl-tryptophan ta hanyar karkatar da bututu mai juzu'i na chromatography na yanzu a ƙarƙashin zafin ɗaki.An gano cewa 0.1% ammonia da aka ƙara a cikin tsarin narkewar ruwa mai ruwa-ruwa ya inganta haɓakar haɓaka.An ga wani babban kololuwa mai fa'ida ga l-tryptophan a lokacin antiseparations.Don ba da bayani, an auna yawan canja wuri na d- da l-enantiomers ta hanyar mu'amala tsakanin matakan biyu.An gano cewa l-tryptophan ya nuna ƙananan yawan canja wurin taro fiye da d-tryptophan.An gabatar da ƙarin tattaunawa don dalilai masu yuwuwa na bambancin canjin kuɗi tsakanin masu haɓakawa.