Kayayyaki

Mesenchymal stem sel (MSCs) an ruwaito su zama tushe mai ban sha'awa don ƙirƙirar ƙwayoyin beta masu maye gurbin.An gane layin embryonic mesenchymal progenitor cell line C3H10T1/2 a matsayin abin ƙira ga MSCs, saboda yuwuwar bambancin sari-sauri.Manufar wannan binciken shine don gano ko ƙwayoyin C3H / 10T1 / 2 suna da yuwuwar bambancewa cikin sel masu samar da insulin (IPCs).Anan, mun bincika kuma mun kwatanta bambancin in vitro na MSCs na bera da ƙwayoyin C3H10T1/2 cikin IPCs.Bayan sel sun sami bambance-bambancen IPC, an gano alamun bambance-bambance ta hanyar immunocytochemistry, jujjuyawar juzu'i-polymerase sarkar dauki (RT-PCR), adadi na ainihin lokaci RT-PCR (qRT-PCR) da gogewar Yamma.An ƙididdige ɓoyewar insulin ta hanyar gwajin immunosorbent mai alaƙa da enzyme (ELISA).Bugu da ƙari kuma, an dasa waɗannan sel daban-daban zuwa cikin berayen masu ciwon sukari da ke haifar da streptozotocin da ayyukansu na rayuwa da aka gwada a vivo.Wannan binciken ya ba da rahoton hanyar 2-mataki don samar da IPCs daga ƙwayoyin C3H10T1/2.Ƙarƙashin ƙayyadaddun yanayin shigarwa na kwanaki 7-8, ƙwayoyin C3H10T1 / 2 sun samar da jikin spheroid masu girma uku (SBs) da ɓoye insulin, yayin da ƙarni na IPCs da aka samo daga MSCs na bera ya buƙaci lokaci mai tsawo (fiye da makonni 2).Bugu da ƙari kuma, waɗannan IPCs da aka samo daga ƙwayoyin C3H10T1/2 an allura su cikin berayen masu ciwon sukari kuma suna inganta glucose na basal, nauyin jiki da kuma nuna gwajin haƙuri na glucose na yau da kullun.Binciken na yanzu ya ba da samfurin in vitro mai sauƙi da aminci don ƙarin bincike kan tsarin da ke haifar da bambance-bambancen IPC na MSCs da maganin maye gurbin cell don ciwon sukari.