Kayayyaki

Tasirin acidosis, alama ce ta biochemical na ƙwayar ƙwayar ƙwayar cuta, akan ci gaban ciwon daji da metastasis yana da rikitarwa.Dukansu pro- da anti-tumorigenic sakamako na acidosis an ba da rahoton kuma an yi amfani da microenvironment na acidic don takamaiman isar da kwayoyi, abubuwan hoto, da gina jiki a cikin ciwace-ciwace.A cikin wannan binciken muna bincikar yadawa da mannewa na sel B16F10 melanoma waɗanda aka ƙera ta halitta don wuce gona da iri na pH-sensing G protein-coupled receptor GPR4.Ta yin amfani da kididdigar haɗe-haɗe da tantanin halitta mun gano cewa GPR4 overexpression yana jinkirta yaɗuwar tantanin halitta kuma ya canza yanayin wuri na hadadden mannewa mai ƙarfi, kamar ganowar phosphorylated focal adhesion kinase (FAK) da paxillin, a pH acidic.An kuma bincika yuwuwar G-protein da hanyoyin sigina na ƙasa waɗanda ke da alhakin waɗannan tasirin.Ta amfani da Rho inhibitor CT04 (C3 transferase), Rho-associated kinase (ROCK) inhibitors Y27632 da thiazovivin, myosin haske sarkar kinase (MLCK) inhibitor staurosporine ko G12/13 inhibitory gini, tantanin halitta ya dawo yayin da hanawa da kuma hanawa. kunna hanyoyin Gq da Gs ba su da wani tasiri ko kaɗan.Gabaɗaya sakamakonmu yana nuna cewa ta hanyar G12/13/Rho hanyar siginar GPR4 tana daidaita haɓakar mannewa mai mahimmanci kuma yana rage yaduwar tantanin halitta da ruffing membrane.