Kayayyaki

Raunin kashin baya na kashin baya yana da rikitarwa ta hanyar jinkirta asarar oligodendrocytes, wanda ke haifar da ci gaba na demyelination na yau da kullum.Sabili da haka, dabarun dasawa don samar da ƙwayoyin layi na oligodendrocyte kuma don haɓaka girman ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta.Binciken da aka yi a halin yanzu ya bincika ko dasawa na ƙwayoyin jijiya na ɗan adam (NSCs) ta hanyar gyare-gyare ta hanyar asali don bayyana Olig2 transcription factor, mai mahimmanci mai kula da ci gaban oligodendrocyte, zai iya inganta farfadowa na locomotor kuma ya inganta myelination a cikin wani nau'in ciwon ƙwayar cuta na bera.HB1.F3 (F3) ) An canza layin NSC na ɗan adam marar mutuwa tare da retroviral vector encoding Olig2, muhimmin mai kula da ci gaban oligodendrocyte.Overexpression na Olig2 a cikin NSCs na ɗan adam (F3.Olig2) ya haifar da kunnawa na NKX2.2 kuma ya jagoranci bambance-bambancen NSC a cikin ƙwayoyin layi na oligodendrocyte a cikin vitro.Gabatarwar Olig2 ya ba da aikin haɓakawa mafi girma, kuma an gano adadin F3.Olig2 NSC mafi girma ta makonni 7 bayan dasawa zuwa cikin kashin baya da aka dasa fiye da na F3 NSC na iyaye.F3.Olig2 NSCs sun nuna ƙaura akai-akai zuwa farar fata, yayin da F3 NSCs galibi an tsare su ne ga al'amuran launin toka ko kuma kewaye da ramukan raunuka.Yawancin F3.Olig2 NSCs masu mamaye fararen fata da aka keɓe sun bambanta zuwa manyan oligodendrocytes.Dasawa na F3.Olig2 NSCs ya kara yawan adadin fararen fata da aka ajiye tare da rage girman rami.Haka kuma, F3.Olig2 grafts muhimmanci ƙara kauri na myelin kwasfa a kusa da axon a cikin kare farin al'amari.A ƙarshe, dabbobi da F3.Olig2 grafts sun nuna haɓakawa a cikin ingancin locomotion na hindlimbs.Transplantation of NSCs genetically modified to different into a oligodendrocytic lineage na iya zama dabarun da ya dace don inganta sakamakon aiki bayan rauni na kashin baya.Binciken da aka yi a yanzu yana nuna cewa za a iya amfani da abubuwan da ke jagorantar yanke shawara na kaddarorin kwayar halitta don samun damar sake fasalin maganin tantanin halitta.