Kayayyaki

γ-Glutamyltranspeptidases (γ-GTs) suna ƙulla γ-glutamyl amide bond na glutathione kuma suna canza ƙungiyar γ-glutamyl da aka saki zuwa ruwa (hydrolysis) ko amino acid mai karɓa (transpeptidation).Wadannan enzymes na ko'ina suna taka muhimmiyar rawa a cikin biosynthesis da lalata glutathione, kuma a cikin xenobiotic detoxification.Anan mun bayar da rahoton tsarin ƙudurin crystal na 3Å na Bacillus licheniformis γ-GT (BlGT) da na hadaddun sa tare da l-Glu.Tsarin X-ray ya tabbatar da cewa BlGT na cikin N-terminal nucleophilic hydrolase superfamily kuma ya bayyana cewa sunadaran yana da buɗaɗɗen rukunin rukunin yanar gizo mai kama da wanda aka ruwaito don enzyme homologous daga Bacillus subtilis, amma ya bambanta da waɗanda aka lura ga ɗan adam γ-GT da don γ-GTs daga sauran ƙwayoyin cuta.Bayanai sun nuna cewa daurin l-Glu yana haifar da sake yin oda na wutsiyar C-terminal na BlGT babban subunit kuma yana ba da izinin gano gungu na ragowar acid waɗanda ke da yuwuwar shiga cikin sanin ion ƙarfe.An yi nazarin rawar da waɗannan abubuwan da suka rage a kan daidaiton kwanciyar hankali na BlGT ta hanyar halayen autoprocessing, aikin enzymatic, sinadarai da denaturation na sabbin halittu guda huɗu na Ala guda.Sakamakon ya nuna cewa maye gurbin Asp568 tare da Ala yana rinjayar duka aikin sarrafawa da kwanciyar hankali na gina jiki.