Kayayyaki

MSMEG_0307 an bayyana shi azaman mai sarrafa rubutu na dangin furotin na AraC kuma yana kusa da arylamine N-acetyltransferase (nat) gene a cikin Mycobacterium smegmatis, a cikin tarin kwayoyin halitta, wanda aka adana a yawancin nau'in mycobacterial na muhalli.Don bayyana aikin furotin na AraC daga nat operon a cikin M. smegmatis, an gano nau'i biyu na palindromic DNA motifs da aka kiyaye ta amfani da bioinformatics kuma an gwada su don haɗakar da furotin ta amfani da gwajin motsi na motsi na electrophoretic tare da nau'i mai mahimmanci na furotin AraC.Mun gano samuwar DNA: hadaddun furotin AraC tare da ɗayan motifs da kuma kasancewar wannan motif a cikin 20 loci a duk faɗin genome na M. smegmatis, yana goyan bayan kasancewar tsarin sarrafa AraC.Don kwatanta tasirin AraC a cikin ka'idojin kwayoyin halitta na nat operon, da kuma samun ƙarin haske game da aikinsa, mun haifar da nau'in mutant na ΔaraC inda aka maye gurbin kwayar halittar araC ta hanyar alamar resi ta hygromycin.Matsayin magana na nat da MSMEG_0308 genes an saukar da su a cikin nau'in ΔaraC idan aka kwatanta da nau'in nau'in daji wanda ke nuna tasirin mai kunnawa na furotin AraC akan maganganun kwayoyin halitta na nat operon.