Kayayyaki

EAAT2 mai jigilar glutamate, lissafin> 90% na ɗaukar glutamate na hippocampal.Kodayake EAAT2 galibi ana bayyana shi a cikin astrocytes, ana samun kashi 10% na ƙwayoyin EAAT2 a cikin tashoshin axon.Duk da ƙananan matakin EAAT2 magana a cikin tashoshi na glutamatergic, lokacin da aka haɗu da yankan hippocampal tare da ƙarancin maida hankali na d-aspartate (wani yanki na EAAT2), tashoshin axon suna tara d-aspartate da sauri kamar astroglia.Wannan yana nuna rashin daidaituwa da ba a bayyana ba tsakanin rarraba furotin EAAT2 da ayyukan jigilar kayayyaki na EAAT2.Ɗaya daga cikin hasashe shine (1) canjin canjin canji na ciki tare da substrate na waje yana da sauri da sauri fiye da karɓar yanar gizo kuma (2) tashoshi suna son heteroexchange saboda manyan matakan glutamate na ciki.Duk da haka, a halin yanzu ba a sani ba ko heteroexchange da ɗauka suna da irin wannan ko daban-daban rates.Don magance wannan batu, mun yi amfani da tsarin da aka sake ginawa don kwatanta ma'aunin dangi na matakai guda biyu a cikin bera da mice.Ɗaukar gidan yanar gizon ya kasance mai hankali ga canje-canje a yuwuwar membrane kuma an motsa shi ta hanyar anions masu yuwuwa na waje a cikin yarjejeniya tare da wanzuwar motsin anion wanda ba a haɗa shi ba.Ta amfani da ƙarshen, muna kuma nuna cewa ƙimar heteroexchange shima ya dogara da yuwuwar membrane.Bugu da ƙari, bayananmu sun ƙara ba da shawarar kasancewar leak ɗin sodium a cikin EAAT2.Ta hanyar haɗa sabon binciken a cikin samfurin mu na baya na karɓar glutamate ta EAAT2, muna hasashen cewa ƙarfin wutar lantarki na musayar ya haifar da ɗaurin Na (+) na uku mai dogaro da wutar lantarki.Bugu da ari, duka gwaje-gwajenmu da kwaikwaiyo suna ba da shawarar cewa ƙimar dangi na haɓakawar yanar gizo da canjin canjin yanayi suna daidai da EAAT2.