Kayayyaki

Rukunin sulfur na baƙin ƙarfe suna aiki azaman masu haɗa nau'ikan sunadaran sunadaran, tare da nau'ikan nau'ikan kwayoyin halitta a cikin ƙwayoyin prokaryotic da eukaryotic.Ƙaddamar da injuna suna haɗa gungu kuma su isar da su zuwa ga ƙwayoyin masu karɓa na ƙarshe a cikin tsari mai tsari.A cikin nau'in kwayar cutar Gram-negative Escherichia coli, tsarin haɗin ginin gungu na ƙarfe-sulfur guda biyu da ake da su, ƙarfe-sulfur cluster (ISC) da sulfur assimilation (SUF), hanyoyin haɗin gwiwa.Mai sarrafa hanyar ISC, IscR, shine nau'in rubutu na nau'in helix-turn-helix wanda zai iya daidaita tarin [2Fe-2S].Yanayin Redox da kasancewar ƙarfe ko sulfur suna daidaita matsayin ligation na gungu na IscR na labile, wanda hakanan ke ƙayyade canji a cikin ƙayyadaddun tsarin DNA na mai gudanarwa: cluster-dauke da IscR na iya ɗaure ga dangin masu tallata kwayoyin halitta (nau'in-1) yayin da Siga mara tari yana gane rukuni na biyu kawai na jeri (nau'in-2).Duk da haka, baƙin ƙarfe-sulfur cluster biogenesis a cikin ƙwayoyin cuta na Gram-positive ba su da kyau sosai, kuma yawancin kwayoyin halitta na wannan rukunin suna nuna ɗaya kawai daga cikin tsarin hada-hadar baƙin ƙarfe-sulfur.Wani abin lura shine keɓantaccen ƙarfe na gram-positive dissimilatory yana rage ƙwayoyin cuta Thermincola potens, inda za'a iya gano kwayoyin halitta daga tsarin biyu, duk da cewa tare da ƙungiyar da ta bambanta daga na kwayoyin Gram-negative.Mun nuna cewa ɗaya daga cikin waɗannan kwayoyin halitta ya haɗa da aikin IscR homolog kuma yana iya shiga cikin tsarin tsarin ƙwayar ƙarfe-sulfur cluster biogenesis a cikin T. potens.Siffar tsari da sinadarai na T. potens da E. coli IscR sun bayyana gine-gine mai kama da kamanceceniya kuma sun bayyana kiyayewar da ba a zata ba na musamman na tsarin nuna wariya na wannan keɓantaccen rukunin masu sarrafa rubutun.