Kayayyaki

Hakazalika da sauran ƙwayoyin cuta na RNA masu inganci, tombusviruses ana yin su ta hanyar hadaddun kwafin kwayar cuta mai kama da ƙwayar cuta (VRC).VRC ta ƙunshi p92 ƙwayar cuta mai lamba RNA-dependent RNA polymerase (RdRp), kwayar cutar p33 RNA chaperone, da kuma sunadaran haɗin gwiwar haɗin gwiwa da yawa.Domin zama RdRp mai aiki bayan fassararsa, yakamata a shigar da furotin mai kwafi p92 a cikin VRC, sannan kunna shi.Mun nuna a baya a cikin gwajin tushen abin yisti mara sel cewa kunna Tumatir bushy stunt virus (TBSV) RdRp yana buƙatar ma'auni (s).A cikin wannan labarin, mun gano sunadaran zafin jiki na salon salula 70 (Hsp70) a matsayin haɗin haɗin haɗin gwiwar da ake buƙata don kunna wani TBSV RdRp mai sake haɗawa da N-a ƙarshe.Bugu da kari, toshe ƙananan tushen kwayoyin halitta na aikin Hsp70 yana hana haɗin RNA ta tombusvirus RdRp a cikin vitro.Bugu da ƙari kuma, mun nuna cewa tsaka tsaki phospholipids, wato, phosphatidylethanolamine (PE) da kuma phosphatidylcholine (PC), inganta RdRp kunnawa a cikin vitro.Sabanin haka, phosphatidylglycerol (PG) yana nuna tasiri mai ƙarfi da rinjaye akan kunnawa in vitro RdRp.Mun kuma nuna cewa PE da PC suna ƙarfafa hulɗar RdRp-viral da-strand RNA [(+) RNA], yayin da PG ke hana ɗaurin RNA hoto mai hoto zuwa RdRp.Dangane da ayyukan stimulatory da inhibitory na nau'ikan phospholipids daban-daban a kunna tombusvirus RdRp, muna ba da shawarar cewa za a iya amfani da abun da ke tattare da ƙwayar ƙwayar ƙwayar cuta ta tombusvirus don daidaita sabon taron VRC yayin kamuwa da cuta. (RdRp), wanda ke da alhakin haɗa zuriyar RNA mai hoto ko bidiyo mai zagaya yanar gizo da sauri a cikin sel masu kamuwa da ƙwayoyin cuta na RNA masu inganci, da farko ba su da aiki.Wannan dabarar mai yiwuwa ta guje wa haɗin RNA mai hoto mai hoto a cikin cytosol wanda zai haifar da sauri ga shigar da martanin rigakafin ƙwayar cuta ta wayar salula mai haifar da RNA.Yayin hada hadaddun kwafin da ke daure membrane, kwayar cutar RdRp tana aiki ta hanyar tsarin da bai cika ba wanda ke sa RdRp ya iya hada RNA.Ta amfani da TBSV RdRp, muna nuna cewa haɗin gwiwar salon salula Hsp70 chaperone da phospholipids tsaka tsaki suna sauƙaƙe kunna RdRp a cikin vitro.Sabanin haka, phosphatidylglycerol (PG) yana da tasiri mai mahimmanci na hanawa akan kunnawa in vitro RdRp da hulɗar RdRp-viral RNA, yana nuna cewa microdomain membranous da ke kewaye da RdRp yana tasiri sosai ga iyawar RNA.Don haka, kunnawar RdRp na hoto ko bidiyo mai zagaya yanar gizo da sauri ya dogara da abubuwan haɗin gwiwar da yawa a cikin ƙwayoyin cuta.