Kayayyaki

Tarin nukiliyar wani nau'i na mutant protein Lamin-A, wanda ake kira Progerin (PG) ko Lamin AΔ50, yana faruwa a cikin Hutchinson-Gilford Progeria Syndrome (HGPS) ko Progeria, cuta mai saurin tsufa.Daya daga cikin manyan alamomin wannan cuta ta kwayoyin halitta shine asarar kitse na sub-cutaneous saboda wani gagarumin lipodystrophy.Mun jawo hankalin mutum PG da GFP -Green Fluorescent Protein a matsayin iko a cikin sel 3T3L1 ta amfani da tsarin lentiviral don nazarin tasirin maganganun PG a cikin ƙarfin bambance-bambancen wannan layin tantanin halitta, ɗayan samfuran adipogenic da aka fi amfani da su.An yi proteomics masu ƙima (iTRAQ) don nazarin tasirin tarin PG.Yawancin sunadaran da aka gyara an inganta su ta hanyar immunoblotting da PCR na ainihi.An yi nazarin aikin mitochondrial ta hanyar aunawa a) aikin basal mitochondrial, b) samar da superoxide anion da c) daidaitaccen mutum na daban-daban na sarkar numfashi. Mun gano cewa wuce gona da iri PG ta hanyar lentivi ral gene bayarwa yana kaiwa ga raguwa a cikin yawan haɓakawa da kuma lahani a cikin iyawar adipogenic idan aka kwatanta da sarrafawa.Binciken ƙididdigar ƙididdiga na ƙididdigewa ya nuna sunadaran sunadaran 181 mahimmanci (p <0.05) an daidaita su a cikin PG-bayyana preadipocytes.Ayyukan mitochondrial sun lalace a cikin sel masu bayyana PG.Musamman, mun gano karuwa a cikin ayyukan hadaddun I da ƙari na Superoxide anion.Shigarwa tare da Reactive Oxygen Species (ROS) scavenger jamiái yana motsa zuwa raguwa a cikin proteolysis na autophagic kamar yadda LC3-II / LC3-I rabo ya bayyana.PG magana a cikin 3T3L1 Kwayoyin inganta canje-canje a yawancin Tsarin Halittu, ciki har da tsarin cytoskeleton, lipid metabolism, calcium. tsari, fassarar, nadawa sunadaran gina jiki da samar da makamashi ta mitochondria.Bayananmu yana ƙarfafa gudunmawar tarin ROS zuwa ga tsufa na tsufa da kuma kafa hanyar haɗi tsakanin rashin aikin mitochondrial da asarar proteostasis a cikin HGPS.