Kayayyaki

Indoleamine 2,3-dioxygenase (IDO1) da tryptophan 2,3-dioxygenase (TDO) sune nau'ikan enzymes daban-daban guda biyu waɗanda ke da nau'ikan rarraba nama daban-daban da kuma matsayin ilimin lissafi, amma duka biyun suna haɓaka jujjuyawar tryptophan zuwa N-formylkynurenine (NFK).An tabbatar da IDO1 a asibiti a matsayin ƙananan ƙwayar ƙwayar ƙwayar ƙwayar cuta don ciwon daji, yayin da bincike na farko ya nuna cewa TDO na iya zama manufa don ciwon daji na rigakafi da cutar neurodegenerative.Mun ƙirƙira babban ƙima mai ƙima don IDO1 da TDO dangane da binciken sinadarai na sabon labari, NFK Green, wanda ke amsawa musamman tare da NFK don samar da kwayar halitta mai kyalli mai launin kore tare da tsayin motsin rai na 400 nm da tsayin iska na 510 nm.Muna ba da kwatancen gefe-da-gefe na farko na adadin masu hanawa da aka buga na IDO1 da TDO kuma mun bayyana cewa madaidaicin IDO1 inhibitor Compound 5l yana nuna mahimmin amsawar giciye tare da TDO, yayin da aka tabbatar da zaɓin dangi na sauran masu hanawa da aka buga.An nuna dacewa da dacewa don babban aikin tantance kima ta hanyar nuna ɗakin karatu na sinadarai 87,000 a cikin tsarin rijiyoyin 384- ko 1536.A ƙarshe, mun nuna cewa ana iya amfani da gwajin don auna ƙarfin sel don daidaita tryptophan da kuma auna ƙarfin salula na IDO1 da masu hana TDO.