Kayayyaki

MES Buffer Yana Shafar Arabidopsis Tushen Apex Zonation da Ci gaban Tushen ta Hannun Ƙarfafa Superoxide a Tushen Apex

A cikin tsire-tsire, haɓakar tushen gashi da tushen gashi ana daidaita su ta hanyar ingantaccen tsarin salula na pH da nau'in iskar oxygen (ROS).MES, 2- (N-morpholino) ethanesulfonic acid a matsayin ɗayan abubuwan buffers mai kyau an yi amfani da shi sosai don matsakaicin buffering, kuma ana tsammanin ya dace da haɓakar shuka tare da maida hankali a 0.1% (w / v) saboda ƙarfin buffer na MES mai jere pH 5.5-7.0 (na Arabidopsis, pH 5.8).Koyaya, rahotanni da yawa sun nuna cewa, a cikin yanayi, tushen yana buƙatar ƙimar pH daban-daban akan saman takamaiman wuraren koli na tushen tushen, wato meristem, yankin canji, da yankin elongation.Duk da cewa tushen koyaushe yana girma akan kafofin watsa labarai mai ɗauke da kwayoyin buffer, kaɗan ba a san tasirin MES akan ci gaban tushen ba.Anan, mun bincika tasirin maɓalli daban-daban na MES buffer ta amfani da tushen girma na Arabidopsis thaliana.Sakamakonmu ya nuna cewa 1% na MES yana hana ci gaban tushen girma, adadin gashin gashi da tsayin meristem, yayin da 0.1% ya inganta ci gaban tushen da tushen kololuwar yanki (yankin da ya tashi daga tushen tushen har zuwa yankin canji).Bugu da ƙari, haɓakar superoxide a cikin tushen tushen ya ɓace a 1% na MES.Waɗannan sakamakon suna ba da shawarar cewa MES yana damun tushen ƙwayoyin cuta na al'ada ta hanyar canza ROS homeostasis a tushen koli.