Kayayyaki

Carbonic anhydrase activators: nazarin kunnawa na farko na isoform sirrin ɗan adam VI tare da amino acid da amines.

Asiri isozyme na ɗan adam carbonic anhydrase (hCA, EC 4.2.1.1), hCA VI, an cloned, bayyana, da kuma tsarkake a cikin wani kwayan cuta tsarin.Ma'auni na motsi don CO (2) amsawar ruwa sun tabbatar da hCA VI don mallaki ak (cat) na 3.4 x 10 (5) s (-1) da k (cat)/K (M) na 4.9 x 10(7)M (-1) s (-1) (a pH 7.5 da 20 digiri C).hCA VI yana da gagarumin aiki na catalytic don maganin ilimin lissafi, na tsari guda ɗaya na girman girman girman isoform CA I ko transmembrane, isozyme CA IX da ke hade da tumor.An nuna jerin amino acid da amines suna aiki azaman masu kunnawa CA VI, tare da ingantaccen tasiri.l-His, l-Trp, da dopamine sun nuna raunin CA VI tasirin kunnawa (K (A) s a cikin kewayon 21-42 microM), yayin da d-His, d-Phe, l-DOPA, l-Trp, serotonin , kuma wasu pyridyl-alkylamines sun kasance masu kunnawa mafi kyau, tare da K(A) s a cikin kewayon 13-19 microM.Mafi kyawun masu kunnawa CA VI sune l-Phe, d-DOPA, l-Tyr, 4-amino-l-Phe, da histamine, tare da K (A) s a cikin kewayon 1.23-9.31 microM.Duk waɗannan masu kunnawa suna haɓaka k (cat), ba su da wani tasiri akan K (M), suna shiga ta haka a cikin ƙimar kayyade matakin a cikin sake zagayowar catalytic, halayen canja wurin proton tsakanin wurin aiki na enzyme da muhalli.