Kayayyaki

A cikin hanyar angiotensin-canzawar enzyme (ACE), mai amfani kuma mafi kyawun buffer shine 80 mmol/L borate buffer a pH 8.2 (37 digiri C).An gano wani lokaci mai rauni a cikin abin da ya faru, kuma ana ba da shawarar shigar da substrate da plasma na mintuna 5 kafin auna motsi.Matsakaicin, N-[3- (2-furyl) acryloyl] -L-phenylalanylglycylglycine (FAPGG), an ƙaddamar da ƙaddamarwa a 1.0 mmol / L kuma ma'aunin ma'auni yana a 345 nm don tabbatar da layi na ma'auni.Hanyar da aka tsara tana amfani da rabon 1:9 na plasma-to-reagent rabo.Madaidaicin kewayon gwajin ya ƙara zuwa kusan 170 U/L, yana wakiltar 25% hydrolysis substrate.An ƙayyade sharar FAPGG ta hanyar auna bambanci tsakanin 1.0 mmol/L FAPGG da mafita na samfur.Ana bincika amincin tsayin raƙuman ruwa ta hanyar lura da ƙimar ƙimar da ake tsammanin ɗaukar maganin FAPGG, kuma karkacewar 1.0-nm daga 345 nm yana canza abin sha da 15.5%.Madaidaicin ƙididdigar ACE a kusan 60 da 100 U/L shine 3.5% da 2.4% a cikin tsari da 2.9% da 2.6% tsakanin tsari, bi da bi.Tazarar magana (2.5th zuwa 97.5th percentiles) shine 41-139 U/L, kuma babu bambanci tsakanin dabi'u ga maza da mata.