Kayayyaki

An bayyana wani tsari mai ƙarfi na immunoassay don ƙayyade progesterone a cikin jini na ɗan adam, wanda ke amfani da chemiluminescence azaman ƙarshen ƙarshen.Progesterone-isoluminol conjugate yana aiki azaman alamar chemiluminescent.Wani juzu'in IgG na maganin antiserum zuwa progesterone-11 alpha-hemisuccinate bovine serum albumin an wuce gona da iri zuwa ganuwar Lumacuvettes P polystyrene.Bayan amsawar dauri, ana wanke cuvettes.Yawan hasken da aka ɗaure a kan oxidation tare da tsarin H2O2-microperoxidase a pH 13 ya ragu tare da haɓaka ƙaddamarwar progesterone kyauta a cikin layin layi akan kewayon 15-1000 pg/tube.Immunoassay na chemiluminescence yana kama da radioimmunoassay dangane da hankali, ƙayyadaddun ƙayyadaddun bayanai, daidaito da daidaito.Gwajin yana ba da fa'idodin saurin sauri da sauƙin sarrafa kansa.Bugu da ƙari, wannan hanyar ba ta ƙunshi yin amfani da radioisotopes ko na mataki na centrifugation ba.