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Dithiothreitol (DTT), CAS: 3483-12-3, a matsayin reagent na kimiyya da aka yi amfani da shi sosai, ana amfani da shi azaman wakili mai ragewa don sulfhydryl DNA, wakili mai ɓoyewa, da rage haɗin disulfide a cikin sunadarai.Wani sabon nau'in ƙari na kore yana taka muhimmiyar rawa wajen inganta aikin baturi.
Dithiothreitol (DTT) wakili ne mai ƙarfi mai ragewa, kuma redicibility ya fi yawa saboda daidaituwar daidaituwar zoben membered shida (mai ɗauke da haɗin disulfide) a cikin yanayin iskar oxygen.Rage haɗin disulfide na yau da kullun ta dithiothreitol ya ƙunshi halayen musanya na sulfhydryl-disulfide guda biyu a jere.Rage ikon dithiothreitol (DTT) yana shafar ƙimar pH, kuma zai iya taka rawar ragewa kawai lokacin da ƙimar pH ta fi 7. Wannan shi ne saboda kawai thiolate anions da aka lalata suna amsawa, yayin da mercaptans ba sa, kuma pKa na ƙungiyoyin mercapto gabaɗaya 8.3.
Dithiothreitol (DTT) ana yawan amfani dashi don rage haɗin disulfide na ƙwayoyin furotin da polypeptides.Yawancin lokaci ana amfani da shi azaman furotin sulfhydryl mai kariya kuma ana amfani dashi a cikin shirye-shiryen rigakafi don hana ragowar furotin cysteine ​​daga samar da disulfides na intramolecular da intermolecular.key.A cikin aiwatar da gano nucleic acid, dithiothreitol (DTT) na iya lalata haɗin gwiwar disulfide a cikin furotin na RNase, ya hana RNase, da sauƙaƙe gudanar da gwaje-gwaje kamar ginin ɗakin karatu na RNA da haɓakar RNA.Hakanan ana amfani da Dithiothreitol (DTT) azaman maganin rigakafi don kare sel da kyallen takarda, azaman mai kare rediyo, da sauransu.
Duk da haka, dithiothreitol (DTT) sau da yawa ba ya iya rage haɗin disulfide da ke cikin tsarin sunadaran (ba zai iya shiga ba).Rage irin wannan haɗin disulfide sau da yawa yana buƙatar denaturation na furotin da farko.
Don hana tasirin batura na lithium-sulfur da haɓaka aikin lantarki na batirin lithium-sulfur, yi ƙoƙarin amfani da dithiothreitol (DTT) azaman wakili mai sausaya don yanke polysulfides masu girma don hana su narkewa.An gauraya Threitol (DTT) cikin takarda mai bangon carbon nanotubes (MWCNTs) don shirya interlayer na DTT.Ana sanya interlayer na DTT tsakanin takaddar lantarki mai kyau da mai raba maɓallin lithium-sulfur rabin-cell, da sulfur mai ɗaukar sararin samaniya na tabbataccen takardar lantarki Game da 2mg/cm2.Sakamakon lura da SEM ya tabbatar da cewa DTT an tarwatsa daidai gwargwado a saman da kuma ɓoyayyen takarda na MWCNTs.Sakamakon gwajin lantarki ya nuna cewa baturin lithium-sulfur tare da tsarin sanwici na DTT yana da ƙayyadadden ƙarfin fitarwa na farko na 1288 mAh/g a ƙimar 0.05C.A karo na farko, ingancin coulombic yana kusa da 100%, kuma takamaiman ƙarfin lokacin caji da fitarwa a 0.5C, 2C, da 4C rates ya kai 650mAh/g, 600mAh/g, da 410mAh/g, bi da bi.Gabatar da tsarin sanwici na DTT na iya yin tasiri sosai ga manyan polysulfides.Yana hana shi ƙaura zuwa na'urar lantarki mara kyau na lithium, ta haka yana hana tasirin shuttle da inganta yanayin sake zagayowar da ingancin coulomb na batir lithium-sulfur.
Ya kamata a lura cewa dithiothreitol (DTT) abu ne mai guba.Misali, a gaban karafa na mika mulki, dithiothreitol (DTT) na iya haifar da lahani ga kwayoyin halitta.A lokaci guda, dithiothreitol (DTT) ) Hakanan zai iya haɓaka yawan guba na wasu mahadi masu ɗauke da arsenic da mercury.Dithiothreitol (DTT) yana da wari mai kauri, wanda zai iya cutar da lafiya saboda shakar numfashi da kuma haduwar fata.Don haka, wajibi ne a kare shi yayin aiki, sanya abin rufe fuska, safar hannu da tabarau, da kuma yin aiki a cikin hurumin hayaki.

Thithreitol (DTT) a matsayin wakili mai shearing a cikin batirin lithium-sulfur
Ana ɗaukar batirin lithium-sulfur a matsayin tsarin baturi mai ƙarfin gaske saboda yawan kuzarinsa da kariyar muhalli.Koyaya, "tasirin jirgin ruwa" na polysulfides yana haifar da rayuwa mara kyau da kuma fitar da kai mai tsanani, wanda ke iyakance aikace-aikacen sa.dalili.
Za'a iya ƙara Thiothreitol (DTT) zuwa baturin azaman wakili mai yanke sassa.Yana iya saurin jujjuya igiyoyin disulfide da sauri a cikin ɗaki, ƙara manyan polysulfides don hana rushewar su, hana tasirin jirgin, da haɓaka aikin lithium electrochemical na batir sulfur.
Dithiothreitol (DTT) azaman ƙari na electrolyte a cikin batura na aluminum / iska
A cikin batirin aluminium / iska na alkaline, dithiothreitol na iya samar da nau'in tsaro mai daidaituwa da tsayayye ta hanyar haɗin haɗin gwiwa mai ƙarfi akan saman aluminum anode, yana hana lalata kansa na anode na aluminum, kuma yadda ya kamata ya inganta aikinsa.