Kayayyaki

Acridine ester (NSP-SA-NHS) Cas199293-83-9 da abubuwan da ke da alaƙa suna da fa'ida sosai alamun chemiluminescent wanda kwanciyar hankali, aiki da azanci ya zarce wasu rediyoisotopes.Acridine esters na iya amsawa tare da sunadaran da ke ɗauke da rukunin amino na farko.A ƙarƙashin yanayi na asali, ana musanya NHS azaman ƙungiyar barin, kuma sunadaran suna samar da ingantaccen haɗin kai tare da acridine ester.Bayan da aka kammala aikin, an cire gishirin acridinium da ya wuce ta hanyar ginshiƙan lalata.

Sunadaran da aka yi wa lakabin Acridine baya buƙatar katalin enzymatic don fitar da haske a gaban alkaline hydrogen peroxide.Ƙimar ƙayyadaddun ƙayyadaddun hasken haske shine cewa a cikin bayani na hydrogen peroxide na alkaline, acridine ester yana kaiwa hari ta hanyar hydrogen peroxide ions don samar da dioxyethane maras kyau tare da tashin hankali, wanda ya kara lalacewa zuwa CO2 da kuma acridone mai farin ciki na lantarki.Lokacin da acridone ya dawo cikin yanayin ƙasa, yana fitar da photons tare da matsakaicin matsakaicin tsayi na 430 nm.Wannan tsari na hasken haske yana da ɗan gajeren lokaci (dukkan tsari yana ɗaukar ƙasa da daƙiƙa 2), kuma makircin ƙaddamarwa dole ne ya ƙara hoto na ciki da na'urar gano hoto;Bugu da ƙari, wannan samfurin kuma zai iya amfani da mai karanta microplate mai aiki da yawa sanye take da autosampler don tattara bayanan haske.Sunadaran, peptides, antibodies, da acid nucleic duk ana iya yiwa alama alama da wannan samfurin.Acridine esters suna fitar da haske da sauri a ƙarƙashin tashin hankali na alkaline hydrogen peroxide, don haka ana iya gano mahaɗan da aka lakafta ta hanyar tattara photons.

Ana amfani da wannan samfurin musamman don: chemiluminescence da immunoassay, nazarin mai karɓa, nucleic acid da gano peptide da sauran bincike.