ONPG CAS: 369-07-3 98.0% Min Fari Zuwa Kashe - Farin Foda
| Lambar Catalog | XD90006 |
| CAS | 369-07-3 |
| Sunan samfur | ONPG(2-Nitrophenyl-beta-D-galactopyranoside) |
| Tsarin kwayoyin halitta | Saukewa: C12H15NO8 |
| Nauyin Kwayoyin Halitta | 301.25 |
| Bayanin Ajiya | 2 zuwa 8 ° C |
| Harmonized Tariff Code | Farashin 2940000 |
Ƙayyadaddun samfur
| Tsaftace (HPLC) | Min.98.0% |
| Bayyanar | Fari zuwa kashe - fari foda |
| Magani(1% a cikin Ruwa) | A bayyane, mara launi zuwa maganin rawaya kadan |
| Abubuwan Ruwa(Karl Fisher) | Max.0.5% |
| Takamaiman jujjuyawar gani [α] D20(c=1, H2O) | - 65.0 ° C zuwa -73.0 ° C |
Tattaunawa akan gwajin ONPG (gwajin β-galactosidase)
An yi ta tambayoyi akai-akai kwanan nan: 1. Me yasa za a iya amfani da gwajin ONPG don bambanta jinkirin fermentation na lactose?2. Me yasa ma'auni na ƙasa ya bayyana cewa ya zama dole a yi amfani da 3% sodium chloride trisaccharide iron (ko trisaccharide iron) don gwajin ONPG?3. Don Vibrio parahaemolyticus, lokacin da ake gudanar da gwajin OPNG, me yasa za a ƙara toluene a hankali bisa ga ma'auni?Menene aikin?
Kamfaninmu ya yi bitar bayanai da yawa, ya taƙaita su, kuma ya raba tare da ku a ƙasa:
Ka'ida: Sunan Sinanci na ONPG shine o-nitrobenzene-β-D-galactopyranoside.Ana iya sanya ONPG hydrolyzed cikin galactose da rawaya o-nitrophenol (ONP) ta β-galactosidase, don haka ana iya gano ayyukan β-galactosidase ta hanyar canjin launi na matsakaicin al'ada.
Lactose shine sukari wanda yawancin ƙananan ƙwayoyin cuta ke buƙatar ganowa.Metabolism nata yana buƙatar enzymes guda biyu, ɗaya shine permease cell, lactose yana shiga cikin sel a ƙarƙashin aikin permease;ɗayan kuma shine β-galactosidase, wanda ke sarrafa lactose zuwa galactose.Lactose da glucose.β-Galactosidase kuma na iya yin aiki kai tsaye akan ONPG don sanya shi cikin galactose da rawaya o-nitrophenol (ONP).Ana iya yin shi a cikin sa'o'i 24, ko da tare da jinkirin lactose.Sabili da haka, yana bayanin sakamakon lura na ɗaukar al'ada 1 daga agar slant da yin amfani da shi a cikin cikakken da'irar a 36 ° C don 1-3h da 24h.Idan an samar da β-galactosidase, zai zama rawaya a cikin 1-3h, idan babu irin wannan enzyme, ba zai canza launi ba a cikin 24h.
Dangane da nau'ikan enzymes guda biyu na sama, ana iya raba microorganisms zuwa nau'ikan masu zuwa:
1 lactose-fermenting (18-24 hours) kwayoyin cuta tare da permease da β-galactosidase P + G +;
2 Jinkirin lactose fermenters (daukar fiye da sa'o'i 24) rashin jin daɗi amma yana da galactosidase: P-G+.
3 Non-lactose fermenters rasa duka permease da galactosidase: P- G-.
Ana iya amfani da gwajin ONPG don bambance kwayoyin lactose-lag-fermenting (P-G+) daga kwayoyin lactose marasa fermenting (PG-), kamar:
1 Bambance maƙarar lactose fermenters (P-G+) da waɗanda ba lactose fermenters (P-G-).
(a) Citrobacter (+) da Salmonella arizonae (+) daga Salmonella (-).
(b) Escherichia coli (+) daga Shigella sonnei (-).
Me yasa aka yi gwajin ONPG ta amfani da al'adun dare akan 3% sodium chloride ferric trisaccharide (iron trisaccharide)?Kamfaninmu ya tuntubi bayanai da yawa, amma babu wata bayyananniyar sanarwa.Sai kawai a kan shafin yanar gizon FDA, an rubuta cewa "Inoculate al'adun da za a gwada su a kan nau'in ciwon sukari guda uku na baƙin ƙarfe agar slants da incubate don 18 h a 37 ° C (ko sauran zafin jiki da ya dace, idan an buƙata) . Na gina jiki (ko wasu) agar slants dauke da 1.0 Hakanan za'a iya amfani da % lactose.ma'anar: an yi amfani da kwayoyin gwajin a kan matsakaicin ƙarfe na trisaccharide kuma an tsara su a 37 ° C na 18h.Abincin agar slants (ko wasu) matsakaici mai ɗauke da 1% lactose shima abin karɓa ne.Saboda haka, an yi la'akari da cewa matsakaicin ƙarfe na trisaccharide ya ƙunshi lactose.Bayan girma na dare, ƙwayoyin cuta sun samar da β-galactosidase mai aiki mai kyau.Yin amfani da irin waɗannan ƙwayoyin cuta, ONPG na iya bazuwa ta β-galactosidase da sauri.Lamarin gwaji ya fi sauri kuma ya fi bayyana.Bugu da kari, juzu'i na toluene da kuma wanka na ruwa na 5 min duka duka don sakin β-galactosidase cikakke ne.
