Kayayyaki

An bincikar hanawar β-carbonic anhydrases (CAs, EC 4.2.1.1) daga fungi na fungi Cryptococcus neoformans (Can2) da Candida albicans (Nce103) tare da jerin 25 rassan aliphatic da aromatic carboxylates.An haɗa isoforms na ɗan adam hCA I da II a cikin binciken don kwatanta.Aliphatic carboxylates gabaɗaya millimolar hCA I da II inhibitors da ƙananan micromolar/submicromolar β-CA inhibitors.Carboxylates na aromatic sune masu hana micromolar na enzymes huɗu amma wasu daga cikinsu sun nuna ƙarancin aikin nanomolar akan ƙwayoyin cuta na fungal.4-Hydroxy- da 4-methoxy-benzoate sun hana Can2 tare da K (I) s na 9.5-9.9 nM.methyl esters, hydroxamates, hydrazides da carboxamides na wasu daga cikin waɗannan abubuwan da aka samo su ma sun kasance masu hana α- da β-CAs masu tasiri a nan.

Parabens suna daga cikin abubuwan da ake amfani da su akai-akai don hana haɓakar ƙwayoyin cuta da tsawaita rayuwar kewayon samfuran mabukaci.Makasudin binciken na yanzu shine don samun fahimta game da metabolism na parabens a cikin ƙwayoyin kansar nono (MCF7) tun da sun nuna ayyukan estrogenic zuwa waɗannan ƙwayoyin cuta kuma an gano su a cikin kyallen ƙwayar nono.An ƙaddara yawan guba na parabens zuwa ƙwayoyin MCF7 ta amfani da ƙididdigar MTT.Hydrolysis na methyl-, butyl da benzyl-paraben zuwa p-hydroxybenzoic acid an bincikar su a cikin al'adar MCF7 Kwayoyin da kuma a cikin salon salula homogenates.An yi nazarin Glucuronidation da sulfoconjugation a cikin MCF7 homogenates, kuma HPLC ta bincikar parabens.An nuna Methyl-paraben ba shi da guba sosai fiye da butyl da benzyl-paraben.Parabens sun kasance kwata-kwata a cikin MCF7 homogenates yayin da p-nitrophenyl acetate, nau'in nau'in nau'i, an yi amfani da hydrolysis.MCF7 cell homogenates ba su bayyana glucuronidation da sulfoconjugation ayyukan zuwa parabens.Mafi girman kwanciyar hankali na parabens na iya bayyana tarin su a cikin ƙwayar nono.