Kayayyaki

Ko da yake an san fluoride don tayar da samuwar kashi, ba a fahimci hanyoyin da ke cikin su ba.Nazarin kwanan nan sun haifar da hanyar Wnt/β-catenin a matsayin babban sigina mai sigina a cikin ilimin halittar kasusuwa.Nazarin mu na farko ya ba da haske game da yuwuwar rawar canonical hanyar Wnt a cikin samuwar kashi na berayen da ke fallasa fluoride na yau da kullun, amma tsarin har yanzu ba a san shi ba.Binciken na yanzu ya ƙaddara shigar da Wnt/β-catenin siginar a cikin bambance-bambancen osteoblastic mai haifar da fluoride.Yin amfani da osteoblasts na bera na farko, mun nuna cewa fluoride yana haɓaka haɓakar osteoblasts da kuma maganganun alkaline phosphate (ALP) da kuma matakan maganganun mRNA na alamomin bambance-bambancen kashi, gami da nau'in I collagen (COL1A1), ALP da osteonectin.Mun ci gaba da samun phosphorylation na fluoride a serine 473 na Akt da serine 9 na glycogen synthase kinase-3β (GSK3β), wanda ya haifar da hana GSK-3β kuma daga bisani tarin nukiliya na β-catenin, kamar yadda aka nuna ta Western blot da immunofluorescence bincike. .Bugu da ƙari, fluoride kuma ya haifar da bayyanar Wnt-wanda aka yi niyya gene runt factor 2 (Runx2).Mahimmanci, ingantaccen tasirin fluoride akan ayyukan ALP da maganganun mRNA na COL1A1, ALP, osteonection da Runx2 an soke su ta DKK-1, mai toshe mai karɓar Wnt/β-catenin.A hade tare, waɗannan binciken suna ba da shawarar cewa fluoride yana haɓaka bambance-bambancen osteoblastic ta hanyar Akt- da GSK-3β-dogara kunna hanyar siginar Wnt/β-catenin a cikin osteoblasts na bera na farko.Abubuwan da muka gano suna ba da sabon haske game da hanyoyin aikin fluoride a cikin osteoblastogenesis.