Kayayyaki

ExPortal protein secretion organelle a Streptococcus pyogenes wani anionic phospholipid ne dauke da membrane microdomain wadatar a cikin Sec translocons da postsecretion protein biogenesis dalilai.Polymyxin B yana ɗaure kuma yana rushe mutuncin ExPortal, yana haifar da ɓarna ɓoyayyiyar guba da yawa.Don samun fahimtar abubuwan da ke tasiri ƙungiyar ExPortal, an gudanar da allon kwayoyin halitta don zaɓar don maye gurbi na polymyxin B mai jurewa ba tare da bata lokaci ba wanda ke nuna ingantaccen amincin ExPortal.Gabaɗaya-genome mai kama da ƙwararrun halittu masu juriya guda 25 da aka bayyana daga maye gurbi ɗaya zuwa huɗu a kowane nau'in halittar ɗan adam wanda aka tattara da farko a cikin rukunin rukunin kwayoyin halitta 10.Gina mutants tare da gogewa ko shigar da mutum ya nuna cewa 7 ainihin kwayoyin halitta suna ba da juriya da haɓaka amincin ExPortal ta hanyar asarar aiki, yayin da 3 ya kasance saboda samun aiki da/ko tasirin haɗin gwiwa.Kwayoyin juriya na asali sun haɗa da mai sarrafa rubutun biosynthesis na lipid biosynthesis, kwayoyin halitta da yawa da ke da hannu wajen siyan abinci mai gina jiki, da nau'ikan kwayoyin halittar da ke cikin martanin damuwa.Membobi biyu na wannan ajin suma suna aiki a matsayin masu kula da ƙwayar cuta ta SpeB cysteine.Binciken maye gurbi mafi yawan lokuta, shafewar nucleotide guda ɗaya a cikin waƙa na ragowar adenine 9 a jere a cikin pstS, yana ɓoye wani ɓangaren babban jigilar jigilar Pi, yana nuna cewa wannan jerin yana aiki azaman canjin ƙwayoyin cuta don sauƙaƙe daidaitawar damuwa.Tare, waɗannan bayanan suna ba da shawarar kasancewar martanin danniya na membrane wanda ke haɓaka ingantaccen amincin ExPortal da juriya ga peptides na antimicrobial cationic.