Kayayyaki

Bambance-bambancen fibroblasts na orbital zuwa cikin adipocytes masu girma da kuma tarawar nama na adipose na gaba an nuna su a cikin ci gaban Graves' orbitopathy (GO).Autophagy yana da hannu a cikin adipogenesis, amma kadan an san game da rawar da autophagy a cikin farawa da ci gaba na GO.Manufar wannan binciken ita ce bincikar tasirin autophagy a cikin pathogenesis na GO.Orbital adipose / connective tissue explants daga marasa lafiya tare da GO da kuma daga batutuwa na al'ada, da kuma warewar fibroblasts na orbital.An jawo Adipogenesis ta amfani da bambance-bambancen matsakaici tare da ko ba tare da hydrogen peroxide ba, kuma an yi amfani da autophagy ta amfani da bafilomycin A1 da Atg5-wanda aka yi niyya short hairpin RNA (shRNA).An gano autophagosomes ta hanyar microscope na lantarki.An yi nazarin bayanin kwayoyin halittar da ke da alaƙa da autophagy da abubuwan da ke da alaƙa da adipogenesis ta hanyar jujjuya sarkar juzu'i-polymerase da/ko bincike ta yamma.An gwada tarin ɗigon lipid ne ta Oil Red O.Bayyanar kwayoyin halittar da ke da alaƙa da autophagy ya kasance mafi girma a cikin kyallen takarda da ƙwayoyin GO fiye da takwarorinsu waɗanda ba GO ba, bi da bi.Interleukin-1β ya karu LC3-II, p62, da furotin Atg7 a cikin kwayoyin GO.An nuna tarin autophagosome a rana ta 4 na adipogenesis kuma an rage ta ranar 10, tare da samuwar lipid droplet.Maganar sunadaran LC3 da p62 sun karu a cikin sa'o'i 48 na bambance-bambance kuma sun ragu a hankali daga ranar 4 zuwa 10. Bafilomycin A1 magani da Atg5 knockdown ta shRNA ya hana tarin lipid droplet tarawa da kuma dakatar da maganganun adipogenic. wadanda ba GO nama da sel ba, suna nuna cewa autophagy yana taka rawa a cikin GO pathogenesis.Gyaran autophagy na iya zama manufa ta warkewa ga GO.