Kayayyaki

MicroRNA (miRNA) biosensor mai mahimmanci mai mahimmanci wanda ke aiki da ruthenium oxide nanoparticle (RuO(2) NP) -wanda aka ƙaddamar da polymerization na 3,3'-dimethoxybenzidine (DB) da miRNA-samfurin jigo na poly insulating (3,3'-dimethoxybenzidine) (PDB) an kwatanta fim ɗin a cikin wannan aikin.An yi biosensor ne ta hanyar haɗaɗɗun monolayer na oligonucleotide kama bincike (CPs) da 4-mercaptoaniline akan lantarki na zinari.Bayan haɓakawa tare da miRNA mai alamar RuO(2) NP, cakuda DB/H (2) O (2) a cikin buffer pH 5.0 0.10 M acetate an yi amfani da shi ga biosensor.RuO(2) NPs suna aiki azaman mai ƙaddamar da polymerization / mai haɓakawa don polymerization na DB.Kuma madaidaitan miRNA na anionic da CPs masu kyauta suna aiki azaman samfuri, suna jagorantar shigar da PDB.Adadin PDB da aka ajiye da ikon sa na rufewa kai tsaye yana da alaƙa da tattara miRNA da aka yi niyya a cikin mafita.Gwaje-gwajen spectroscopic impedance Electrochemical sun nuna cewa alaƙar juriya-canjawa juriya ta madaidaiciya daga 6 .0 fM zuwa 2.0 pM an samu bayan 60 min na shiryawa a cikin cakuda DB/H (2) O (2).Ba a sami haɗin kai tsakanin pre-miRNA da balagagge miRNA da ɗan ƙaramin haɗe-haɗe tsakanin dangin miRNA masu alaƙa ko da a matakan da ba su dace da tushe guda ɗaya ba.Wannan biosensor na tushen impedance yana ba da zaɓi mai ban sha'awa don bayanin martabar miRNA kuma yana iya ba da damar haɓaka tsarin bayanan miRNA mai ɗaukar nauyi mai ɗaukar nauyi.