Kayayyaki

Multitarget mahadi ana ƙara da ake bi domin tasiri magani na hadaddun cututtuka.Anan, mun bayyana ƙira da haɗin wani sabon aji na shogaol-huprine hybrids, wanda ake zargin ya kai hari da maƙasudin maƙasudi da yawa waɗanda ke cikin cutar Alzheimer.An gwada hybrids a cikin vitro don aikin hanawa akan mutum acetylcholinesterase da butyrylcholinesterase da aikin antioxidant (ABTS.+, DPPH da Folin-Ciocalteu assays), kuma a cikin ƙwayoyin Escherichia coli marasa lafiya don su Aβ42 da tau anti-aggregating ayyukan.Hakanan, an tantance shigar kwakwalwarsu (PAMPA-BBB assay).Kodayake hybrids ba su da ƙarfi masu hana AChE ko masu maganin antioxidant kamar iyayen huprine Y da [4] -shogaol, bi da bi, har yanzu suna nuna ƙarfin anticholinesterase da ayyukan antioxidant kuma sun fi ƙarfin Aβ42 da tau anti-aggregating agents fiye da mahaifa mahadi.Gabaɗaya, shogaol-huprine hybrids suna fitowa a matsayin kwakwalwa mai ban sha'awa wanda zai iya jujjuya maganin cutar Alzheimer.

Abubuwan da aka samo daga nau'ikan polarity daban-daban da aka samo daga sassa daban-daban na shuka (tushen, ganye, furanni da 'ya'yan itace) na Seseli rigidum an yi nazarin su ta hanyar gwaje-gwajen antioxidant daban-daban: DPPH da ABTS radical scavenging aiki, ta hanyar rage yawan ikon da ta hanyar jimlar abun ciki na flavonoids da polyphenols. .Mahimman mai na duk sassan shuka sun nuna halayen antioxidant masu rauni.Matsakaicin maida hankali na hanawa na abubuwan da aka gwada, akan ƙwayoyin cuta Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, da fungi Candida albicans da Aspergillus niger shine 0.01-1.50 mg / ml da na microbicidal 0.02-3.00 mg / ml.A cikin hulɗa tare da cholinesterase, duk mahimman mai sun tabbatar da tasiri a matsayin masu hanawa.Mafi girman kashi na hanawa da ɗan adam da doki cholinesterase an nuna su ta tushen mai mahimmanci (38.20% da 48.30%, bi da bi) tsakanin mai, da tushen hexane cire (40.56% da 50.65% bi da bi).GC, GC-MS da sararin kai/GC-MS ne suka gano mahimman mai da abubuwan da ba su da ƙarfi na duk sassan shuka.Kididdigar kididdiga na tarin sakamakon ya nuna cewa tushen mahimmancin mai ya bambanta da mahimmanci daga mahimman mai na sauran sassan shuka.Yin la'akari da duk ayyukan da aka yi nazari, tushen tushen hexane ya nuna mafi kyawun kaddarorin gabaɗaya.Ta hanyar babban aikin chromatography na ruwa haɗe zuwa babban ƙayyadaddun abubuwan spectrometry, an gano mafi yawan abubuwan 30 a cikin tsantsa na polarity daban-daban.Kasancewar abubuwan da aka gano an danganta su da takamaiman ayyukan nazarin halittu, don haka zayyana mahadi masu yuwuwar alhakin kowane aikin da aka nuna.