Kayayyaki

Ko da yake ba a fahimci ilimin ilimin ilimin cututtuka na intervertebral disc degeneration ba, hanya ɗaya don hana wannan tsari na iya zama hana apoptosis.A cikin binciken na yanzu, an bincika tasirin anti-apoptotic na carboxymethylated chitosan (CMCS) a cikin ƙwayoyin sel pulposus (NP) tare da manufar haɓaka rayuwar ƙwayoyin diski.Kwayoyin NP na beraye sun keɓanta kuma an haɓaka su a cikin vitro, kuma an yi amfani da hydrogen peroxide (H2O2) don gina samfurin apoptosis cell NP.An tantance iyawar tantanin halitta tare da kit-8 kit-8.An yi nazarin rabon sel apoptotic ta hanyar annexin V-fluorescein isothiocyanate (FITC) da propidium iodide (PI) bincike mai sau biyu, kuma Hoechst 33342 tabo ya lura da ilimin halittar jiki.An kimanta yuwuwar mitochondrial membrane na sel NP ta rhodamine 123 fluorescence tabo.Reverse transcription (RT)-ƙididdigar sarkar polymerase (qPCR) an yi don auna matakan mRNA na inducible nitric oxide synthase (iNOS), caspase-3, lymphoma B-cell (Bcl)-2, nau'in II collagen da aggrecan.An gudanar da bincike don gano matakan furotin na iNOS da Bcl-2.Sakamakon annexin V-FITC/PI da Hoechst 33342 sakamakon tabo sun nuna cewa CMCS ya iya hana ƙwayoyin NP daga apoptosis ta hanyar dogaro da kashi.Rhodamine 123 tabo ya fayyace cewa CMCS ya rage rashin lahani na yuwuwar membrane na mitochondrial a cikin ƙwayoyin NP da aka yi wa H2O2 magani.An gano raguwar caspase-3 da ƙara yawan ayyukan Bcl-2 a cikin ƙwayoyin NP da aka yi wa CMCS ta hanyar RT-qPCR da bincike na ɓoyayyiyar yamma.CMCS kuma ya inganta haɓakawa da ɓoye nau'in collagen na II da aggrecan a cikin ƙwayoyin NP da aka yi wa H2O2 magani.An nuna CMCS don yin tasiri wajen hana mutuwar kwayar cutar apoptotic a cikin vitro, yana nuna yiwuwar amfani da wannan hanyar warkewa a cikin daidaita lalata diski.