Kayayyaki

Cysteine ​​​​(Cys) yana taka muhimmiyar rawa a yawancin tsarin ilimin lissafi na ƙwayoyin eukaryotic kuma gano shi a cikin sel yana da mahimmanci.Duk da haka, glutathione (GSH), homocysteine, N-acetyl-L-cysteine ​​​​da sauran thiols suna kawo cikas sosai ga gano Cys.Musamman, GSH yana tsoma baki sosai tare da ganowar Cys salula (30-200 μM) saboda yawan ƙwayar ƙwayar ƙwayar cuta (1-10 mM).A cikin wannan aikin, an gabatar da bincike-bincike mai walƙiya (HOTA) don gano Cys.Wannan binciken yana da kyakkyawar fahimta da zaɓi mai gamsarwa don ganowar Cys ta salula: tare da ƙari na 200 μM Cys, ƙarfin haske na binciken (10 μM) ya haɓaka ninki 117 kuma an ƙididdige iyakar ganowa zuwa 13.47 μM, wanda shine ƙasa fiye da salon salula Cys maida hankali;Hakanan binciken ya zaɓi 30-200 μM cysteine ​​akan 1-10 mM glutathione.Sakamakon haka, an yi gwajin hoton tantanin halitta tare da bincike HOTA.Bugu da ƙari, sakamakon thiol-blocki ng da haɗin GSH na hana gwaje-gwaje sun tabbatar da cewa fitar da kwayar cutar ta cikin salula ya samo asali ne daga hulɗar tsakanin Cys da HOTA.