Kayayyaki

Ciwon hanta mara barasa (NAFLD) yana da nau'ikan nau'ikan cututtukan cututtukan da ke kama da ƙarancin steatosis wanda ke da alaƙa da riƙewar lipids a cikin ƙwayoyin hanta zuwa steatohepatitis (NASH) yana nuna tarin mai, kumburi, balloon da lalata hanta, da fibrosis.Daga qarshe, steatohepatitis na iya haifar da cirrhosis na hanta da ciwon hanta. -1,4-dihydrocollidine (DDC) mai dauke da abinci.RNA-Seq nazarin maganganun kwayoyin halitta, nazarin furotin da bayanin martaba an yi amfani da su don gano nau'ikan kwayoyin halitta / sunadaran da aka bayyana daban-daban da matakan metabolite na samfuran hanta na linzamin kwamfuta akan DDC-jiyya.Binciken hanyar ya nuna canjin arachidonic acid (AA) da S-adenosylmethionine (SAMe) metabolism akan sauran hanyoyin.Don fahimtar canje-canje na rayuwa na arachidonic aci d metabolism a cikin hasken bayyanar cututtukan cututtuka an haɓaka samfurin motsi na wannan hanyar kuma an inganta su bisa ga matakan metabolite.Daga bisani, an yi amfani da samfurin don yin nazari a cikin tasirin silico na yiwuwar magungunan miyagun ƙwayoyi don steatohepatitis. Mun gano AA / eicosanoid metabolism kamar yadda ya damu sosai a cikin ƙananan ƙwayoyin DDC ta amfani da haɗin gwaninta na gwaji da kuma a cikin tsarin silico.Binciken mu na hanyar rayuwa ta AA / eicosanoid yana nuna cewa 5-hydroxyeicosatetraenoic acid (5-HETE), 15-hydroxyeicosatetraenoic acid (15-HETE) da prostaglandin D2 (PGD2) suna cikin damuwa a cikin mice DDC.Mun ƙara nuna cewa za'a iya amfani da samfur mai ƙarfi don tsinkayar ƙimar sauye-sauyen rayuwa bisa ga bayanan da aka rubuta a cikin mahallin da ke da alaƙa da cuta.Bugu da ƙari, SAME metabolism an gano yana da damuwa saboda jiyya na DDC.Yawancin kwayoyin halitta da kuma wasu metabolites na wannan rukunin suna nuna bambance-bambance tsakanin A/J da C57BL/6J a daya hannun da PWD/PhJ a daya bangaren.