Kayayyaki

Mun riga mun ci karo da matakai na tsari inda dihydrotestosterone (DHT) ya yi tasiri na hanawa akan kwayar cutar kwayar cutar parathyroid hormone (PTHrP) ta hanyar mai karɓar isrogen (ER) α, amma ba mai karɓa na androgen (AR), a cikin ciwon nono MCF-7 Kwayoyin. .Anan, mun bincika ko irin wannan hulɗar ligand-nukiliya mai karɓa (NR) tana cikin ƙwayoyin cutar kansa ta prostate LNCaP.Na farko, mun tabbatar da cewa ƙwayoyin LNCaP sun bayyana adadi mai yawa na AR a matakan da ba su da kyau na ERα/β ko mai karɓar progesterone.Dukansu kashewa na PTHrP da kunna prostate-takamaiman kwayoyin antigen an lura dasu bayan gudanar da zaman kanta na 17β-estradiol (E2), DHT, ko R5020.Daidai da ra'ayin cewa LNCaP AR ya rasa ƙayyadaddun ligand ɗin sa saboda maye gurbi (Thr-Ala877), gwaje-gwaje tare da siRNA da aka yi niyya ga kowane NR ya nuna cewa AR ta keɓance rawar mai shiga tsakani na ƙa'idodin dogaro da hormone, wanda ke da alaƙa koyaushe. da makaman nukiliya translocatio n na wannan mutant AR.Binciken ƙayyadaddun ƙayyadaddun ƙayyadaddun kwayoyin halitta ta DHT, E2, ko R5020 sun bayyana cewa fiye da rabin kwayoyin halittar AR (Thr-Ala877) sun mamaye cikin ƙwayoyin LNCaP.Musamman ban sha'awa, mun fahimci cewa AR (nau'in daji [wt]) da AR (Thr-Ala877) suna da alhakin hulɗar E2-AR.Gwaje-gwajen microscopy na fluorescence sun nuna cewa duka EGFP-AR (wt) da EGFP-AR (Thr-Ala877) an keɓance su ne kawai a cikin tsakiya bayan E2 ko DHT magani.Bugu da ƙari kuma, ƙididdigar masu rahoto sun bayyana cewa wasu ƙwayoyin cutar kansa sun nuna alamar E2-AR (wt) mai kama da wannan a cikin ƙwayoyin LNCaP.A nan muna ƙaddamar da kasancewar hulɗar cuɗanya tsakanin wt AR da E2 a cikin wasu ƙwayoyin cutar kansa na hormone.