Kayayyaki

An ƙirƙiri ingantaccen aptasensor wanda aka yi amfani da graphene oxide (GO) azaman mai nuna alama ga duka abubuwan da ke haifar da siginar siginar electrochemiluminescence (ECL).An ƙirƙira aptasensor ta hanyar haɗa kai da kai na binciken ECL na wani thiolated adenosine triphosphate daure aptamer (ABA) mai alamar Ru complex (Ru(bpy)3(2+) abubuwan da suka samo asali) akan saman nanoparticle na gwal (AuNP) wanda aka gyara gilashin carbon. electrode (GCE).ABA immobilized uwa AuNP gyara GCE iya karfi adsorb GO saboda karfi π-π hulɗa tsakanin ABA da graphene oxide;ECL quenching na Ru hadaddun yana faruwa saboda canjin makamashi da canja wurin lantarki, da kuma karuwar juriya ta hanyar canja wurin lantarki (Ret) na lantarki.Duk da yake a gaban adenosine triphosphate (ATP), ABA ya fi son samar da ABA-ATP bioaffinity complexes, wanda ke da rauni dangantaka da graphene oxide da kuma kiyaye graphene oxide daga electrode surface, don haka ba da damar ng da ECL siginar haɓakawa, da kuma tare da raguwar Ret.Saboda babban ingancin kashewar ECL, tsari na musamman, da kaddarorin lantarki na graphene oxide, ƙarfin Ret da ECL tare da logarithm na taro na ATP sun kasance madaidaiciya a cikin kewayo mai fa'ida daga 10 pM zuwa 10 nM tare da ƙarancin ganowa mara nauyi na 6.7 pM zuwa 4.8 pM, bi da bi.Aptasensor da aka tsara ya nuna kyakkyawan sakewa, kwanciyar hankali, da zaɓi na musamman, kuma ana iya bambanta ATP da kyau daga analogues.Mafi mahimmanci, wannan ingantaccen dabarun ECL aptasensor dangane da GO yana aiki duka azaman electrochemical da ECL siginar siginar gabaɗaya kuma ana iya faɗaɗa shi cikin sauƙi zuwa sauran abubuwan da suka shafi ilimin halitta.