4-Nitrophenyl-alpha-D-galactpyranoside CAS:7493-95-0 99%
| Lambar Catalog | XD900018 |
| Sunan samfur | 4-Nitrophenyl-alpha-D-galactpyranoside |
| CAS | 7493-95-0 |
| Tsarin kwayoyin halitta | Saukewa: C12H15NO8 |
| Nauyin Kwayoyin Halitta | 301.25 |
| Bayanin Ajiya | -15 zuwa -20 ° C |
| Harmonized Tariff Code | Farashin 2940000 |
Ƙayyadaddun samfur
| Bayyanar | Fari zuwa Orange zuwa Koren foda zuwa crystal |
| Assay | 99% |
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) wani abu ne na wucin gadi na 4-nitrophenyl (pNP) glycopyranoside don gano ayyukan α-galactosidase.Adadin pNP da aka saki ya karu sosai lokacin da aka yi amfani da 4-Nitrophenyl α-D-galactpyranoside azaman ma'auni.
Amfani da4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, theamountofliberatedpNPdaga4-Nitrophenyla-D-galactopyranoside yana da mahimmanci ga girma daga theotherpNP-glycopyranosidespH4.0inda abin da ya shafi aikin gyarawa. yl (pNP) glycopyranosidessarficial substrates yana nuna cewa an sami 'yanci galactoseresidues daga4-Nitrophenyla-D-galactopyranosidemainlyatpH4.0, iethepHlevel inda ake samun ƙofa yana raguwa sosai.
Substrate na α-D-galactosidase (α-D-Galactosidase).Yana ɗaure zuwa E. coli mai ɗaukar furotin lactose.Matsakaici don α-D-galactose.
An haɓaka hanyar da sauri don tantance ayyukan lytic na magungunan antimicrobial akan yisti da fungi.Binciken ya dogara ne akan sakin enzyme na ciki, maltase (alpha-glucosidase).Ayyukan maltase da aka saki an auna su da launi ta hanyar samar da p-nitrophenol daga p-nitrophenyl-alpha-D-glucopyranoside (PNPG).An auna aikin lytic na mahadi na ƙwayoyin cuta daban-daban akan ƙwayoyin yisti ko germinating spores na filamentous fungi.Za a iya gano ayyukan anti-yisti na Lytic a cikin 20 min incubation a digiri 30 C a kan Saccharomyces cerevisiae, Candida albicans, da Cryptococcus neoformans.Ayyukan anti-fungal na Lytic sun bayyana bayan sa'o'i 2 na shiryawa a digiri 30 na C akan germinating spores na Aspergillus niger da Botrytis cinerea.Dukan sel na yisti ko fungi ba su samar da isasshen PNPG a cikin sa'o'i 3 a digiri 30 don samar da canjin launi mai iya ganowa.Ayyukan lytic na enzymes (misali, Lyticase), maganin rigakafi (misali, Amphotericin B), da nau'in ƙwayoyin cuta masu haifar da ƙwayoyin cuta an gano su ta amfani da gwajin.An daidaita gwajin rigakafin yisti zuwa tsarin microtiter mai rijiyar 96.Dukkanin gwaje-gwajen biyu sun ba da saurin ganowa, mai hankali, da kuma sake sakewa na lytic anti-yiast da aikin rigakafin fungal.
