Kayayyaki

Tsarin 3D da ake iya faɗi na DNA mai madauri biyu ya sa ya dace da dacewa azaman samfuri don ƙirar ƙasa sama na wuraren aiki na tushen nucleic acid.Anan muna bincika yin amfani da duplex DNA na 14mer a matsayin maƙasudi don daidaitaccen matsayi da tsinkaya na ayyuka masu ƙarfi.Ganin kasancewar ƙungiyar imidazole na tushen histidine a ko'ina cikin gano furotin da abubuwan catalysis, an bincika duplexes iri ɗaya na histidine.Haɗa histamine zuwa C5 na tushen thymine ta hanyar haɗin amide, yana ba da damar daidaitawa na aikin imidazole a cikin babban tsagi.Ana bincika hulɗar juna tsakanin imidazole da duplex da tasirinsa akan imidazolium pKaH ta hanyar sanya thymine guda ɗaya da aka gyara a wurare daban-daban guda hudu a tsakiyar 14mer biyu helix.Yin amfani da NMR da haɓakar ƙwayoyin ƙwayoyin cuta marasa ƙarfi, an kafa wani tsarin tsarin da ya shafi samuwar haɗin hydrogen tsakanin imidazole da Hoogste a gefen ginshiƙin guanine na maƙwabtan GC guda biyu maƙwabta.Tushen yana ba da gudummawa ga daidaitawa da narkewar zafin jiki na 6°C kuma shine mabuɗin don daidaita pKaH na ƙungiyar imidazolium.An siffanta fasali na gaba ɗaya, abubuwan da ake buƙata da kuma jigon sabon tsarin tsarin pKaH.