Kayayyaki

APS-5 ne mai chemiluminescent substrate bisa 9,10-dihydroacridine, wanda aka fi amfani da shi don gano ELISA na mahadi tare da alkaline phosphatase AP conjugates;9,10-dihydroacridine a cikin tsarin APS-5 Tsarin hydroacridine zai iya fitar da haske ci gaba, da ƙarfi da inganci a ƙarƙashin aikin AP, kuma APS-5 ya dace sosai don gwajin aikin phosphatase da phosphatase enzyme immunoassay.

A matsayin bincike mai haske, ana amfani da shi a cikin binciken kwakwalwan kwayoyin halitta.Ana yiwa alamar da martani tare da acridan (9,10-dihydroacridine) azaman sinadari da alkaline phosphatase, yana samar da ingantaccen chemiluminescence mai ƙarfi.Yana ba da mafi girman hankali da sauƙin amfani don haɗakarwar alkaline phosphatase yayin gano chemiluminescent.Ana gano alkaline phosphatase a cikin ƙasa da 10-19 mol, kololuwa cikin sauri don rage lokacin ganowa da haɓaka kayan aiki, kuma gangaren madaidaicin madaidaicin layi yana ƙulla tare da logarithmic daidai da 1.0.Daya yawa ko fiye na enzyme yana samar da adadi ɗaya ko fiye na luminescence, ci gaba da haskakawa-ba mai buƙata sosai akan lokacin tantancewa.Ana iya karanta ƙarfin hasken haske daga madaidaicin daidaitawar layin da aka ƙirƙira a kowane lokaci, kuma sakamakon bincike ba shi da hankali ga zafin jiki a cikin kewayon 22°C - 35°C, yana rage daidaiton da ake buƙata don sarrafa zafin jiki.

amfani:

1 Babban hankali - ana iya gano alamun furotin a ƙarƙashin 5pg;

2 Babban ƙarfin haske - ana iya kaiwa ga kololuwar haske a cikin ɗan gajeren lokaci;

3 Ci gaba da kuma barga luminescence - ta luminescence tsari ba a shafa da zazzabi tsakanin 25-35 ℃, kuma baya bukatar zafin jiki kula.

Kwatankwacin aikace-aikacen abubuwan da aka saba amfani da su na luminescent APS-5 (193884-53-6) da AMPPD (122341-56-4)

Dukansu APS-5 (193884-53-6) da AMPPD (122341-56-4) sune kemiluminescent substrates don alkaline phosphatase, kuma duka biyun ana kiransu ultra-high sensitivity detection reagents, don haka waɗannan reagents guda biyu suna cikin ganowa Wanne ya fi kyau kuma wanne ya fi muni?Yadda za a zabi lokacin amfani da shi?

A gefe guda, lokacin da aka yi amfani da APS-5 azaman tushen gano abubuwan gano phosphatase na alkaline, ƙwarewar ganowa zai iya kaiwa 10-19 taro na molar, lokacin haɓaka launi zai iya kaiwa matsakaicin kololuwa cikin kusan daƙiƙa 10, kuma haske yana ɗaukar dogon lokaci. lokaci.A wannan yanayin, ana iya cewa APS 5 yana da kyawawan kaddarorin.Koyaya, APS-5 yana iyakance ne a cikin zafin aikace-aikacen, kuma yanayin yanayin yanayinsa ya fi dacewa ƙasa da 20'C.A gefe guda, lokacin da aka yi amfani da AMPPD azaman abin ganowa don alkaline phosphatase, ana kuma kiran ƙimar ganewar ultra-high sensitivity, amma ba zai iya kaiwa matakin APS 5. Lokacin haɓaka launi ya kai matsakaicin matsakaicin kusan 30 seconds. , da kuma tsawon lokacin kyalli.AMPPD yana da kyau a cikin abubuwan da ke sama, amma yana da ƙasa da APS5, amma yanayin yanayin yanayinsa bai kai APS5 ba, kuma zazzabi na 2--8C ya fi APS-5 sauƙi.A taƙaice, ko da yake APS-5 ya zarce AMPPD a cikin daidaiton ganowa, yana asara cikin kwanciyar hankali.Yadda za a zabi shi ya dogara da takamaiman bukatun ganowa.

1. Gabatarwa na APS-5 (193884-53-6):

Sunan Ingilishi: APS-5

Lambar CAS: 193884-53-6

Tsarin kwayoyin halitta: C21H15ClNO4PS Na2

Nauyin Kwayoyin: 489.819

Bayyanar: kashe-fari m foda

Abun ciki: ≥98%

Bayani: 1g, 5g, 10g

Yanayin ajiya: -20 ℃ kiyayewa

(4-Chlorophenylmercapto) (10-methyl-9,10-dihydroacridine methylene) phosphate disodium gishiri, da ake magana a kai a matsayin APS-5, wani chemiluminescent reagent ne kuma ana amfani da shi azaman chemiluminescent substrate.Yana ba da mafi girman hankali da sauƙin amfani don haɗakarwar alkaline phosphatase yayin gano chemiluminescent.Ana yiwa alamar da martani tare da acridan (9,10-dihydroacridine) azaman sinadari da alkaline phosphatase, yana samar da ingantaccen chemiluminescence mai ƙarfi.APS-5 shine ingantaccen reagent don tantance ayyukan phosphatase ta hanyar tantancewar bayani da phosphatase enzyme immunoassays.A matsayin luminescent substrate, APS-5 yana da babban ganewar ganewa da ɗan gajeren lokacin amsawa.

2. Cikakken gabatarwar AMPPD (122341-56-4)

Sunan Ingilishi: AMPPD

Lambar CAS: 122341-56-4

Tsarin kwayoyin halitta: C18H23O7P

Nauyin kwayoyin halitta: 382.344781

Bayyanar: farin foda

Tsafta: ≥97%

Bayani: 1g, 5g, 10g

Yanayin ajiya: -20 ℃ kiyayewa

Kariya: Dole ne ya bushe don guje wa narke da yawa da lyophilization