Kayayyaki

CTP: phosphocholine cytidylyltransferase α (CCTα) wani enzyme ne na nukiliya wanda ke haifar da matakan iyakacin iyaka a cikin hanyar CDP-choline don haɗin phosphatidylcholine (PC).Kunna lipid na CCTα yana haifar da jujjuyawar sa zuwa ambulaf ɗin nukiliya da faɗaɗa hanyar sadarwa ta intranuclear membrane da ake kira nucleoplasmic reticulum (NR) ta hanyar hanyar da ta haɗa da nakasar membrane.Hakanan ana buƙatar lamin nukiliya don kwanciyar hankali da haɓaka NR, amma ko wannan tsari na musamman, ko lamina na nukiliya gabaɗaya, ana buƙata don haɗawar PC ba a sani ba.Don bincika wannan dangantakar, RNAi ta lalata lamina ta nukiliya ko kuma ta rushe ta hanyar bayyanar cututtuka na Hutchinson-Gilford progeria (HGPS) mutant lamin A (progerin), kuma an bincika tasirin CCTα da choline metabolism.SiRNA-tsakanin shiru na lamin A/C ko lamin B1 a cikin sel CHO don rage NR ba shi da wani tasiri akan haɗin PC, yayin da ƙwanƙwasa sau biyu ba na musamman ya hana hanya.Tabbatar da ƙaramar aikinsa a cikin haɗin PC, kawai 10% na choline/ethanolamine phosphotransferase mai wuce gona da iri an gano shi a cikin NR.A cikin ƙwayoyin CHO, CCTα ya kasance nucleoplasmic kuma an haɗa shi tare da GFP-progerin a cikin folds na nukiliya da invaginations;duk da haka, HGPS fibroblasts sun nuna rarraba mara kyau na CCTα a cikin cytoplasm da ambulan nukiliya wanda ke tare da raguwa na 2-ninki a cikin haɗin PC.Duk da sauye-sauyen da aka samu, gwaje-gwajen alamar choline sun nuna cewa ayyukan CCT ba su da tasiri, kuma an gano hana haɗin PC zuwa rage yawan ayyukan da ke dauke da hemicholinium-sensitive choline transporter.Mun kammala cewa CCTα da lamins suna ba da haɗin kai musamman don samar da NR, amma tsarin gaba ɗaya na ambulaf ɗin nukiliya yana da ƙaramin tasiri akan ayyukan CCT da haɗin PC.