Kayayyaki

Samar da salon salula na deoxynucleoside triphosphates (dNTPs) yana da mahimmanci don kwafi da gyara DNA.A cikin wannan binciken, mun bincika rawar CMP / UMP kinase (CMPK), wani enzyme yana haifar da samuwar CDP, a cikin gyaran DNA.Knockdown na CMPK yana jinkirta gyaran DNA yayin dawowa daga lalacewar UV a cikin ƙwayoyin da ba su da jini amma ba a cikin sel ba tare da rashi na jini ba.Exogenous wadata na cytidine ko deoxycytidine yana sauƙaƙe gyaran DNA wanda ya dogara da CMPK a cikin ƙwayoyin da ba su da jini, yana nuna cewa haɗin dCDP ko CDP yana ƙayyade ƙimar gyaran.Koyaya, ƙwanƙwasa CMPK baya shafar tsayayyen matakin dCTP a cikin ƙwayoyin da ba su da magani.Daga nan mun sami ganowar CMPK a wuraren lalata DNA da hadadden samuwarsa tare da Tip60 da ribonucleotide reductase.Bincikenmu ya nuna cewa ana buƙatar N-terminal 32-amino-acid na CMPK don ɗaukar shi zuwa wuraren lalata DNA ta hanyar dogaro da Tip60.Sake bayyana nau'in daji amma ba N-terminus da aka goge CMPK yana dawo da ingancin gyaran DNA a cikin ƙwayoyin ƙwanƙwasa CMPK.Mun ba da shawarar cewa takamaiman dCDP na musamman ta hanyar CMPK yana ba da hanya don sauƙaƙe gyaran DNA a cikin ƙwayoyin da ba su da magani.