Kayayyaki

Sa ido kan ilimin harhada magunguna baya ga pharmacokinetics yana ɗaya daga cikin dabarun keɓance magungunan mycophenolate mofetil.Manufar wannan binciken shine don haɓaka hanyoyin chromatography-tandem mass spectrometry (LC-MS/MS) don kimanta magunguna da magunguna na mycophenolic acid (MPA).An ƙaddara abubuwan da aka tattara na mycophenolic acid glucuronide (MPAG), mycophenolic acid acyl-glucuronide, da MPA da MPAG wanda ba a ɗaure ba, kuma an ƙididdige ayyukan inosine-5'-monophosphate dehydrogenase ta hanyar auna yawan adadin xanthosine-5'-monophosphate (XMP). ) da kuma adenosine-5'-monophosphate na intracellular bayan shiryawa na kwayoyin halitta na jini mononuclear cell (PBMC) lysates.An yi amfani da ginshiƙin Mastro(TM) mara ƙarfe da ƙirar gradient guda biyu don haɓaka iyakar ƙididdigewa na XMP zuwa 0.1 μM.A cikin kewayon maida hankali na MPA na asibiti, layin daidaitawa, daidaitaccen tsaka-tsaki da tsaka-tsaki da daidaito sun gamsu da ƙa'idodin Hukumar Abinci da Magunguna ta Amurka.Matsakaicin MPA a cikin marasa lafiya na hematopoietic stem cell transplant (HSCT) da aka ƙaddara ta hanyar nazarin enzyme da kuma hanyar LC-MS / MS na yanzu sun nuna kyakkyawar dangantaka (r (2) = 0.95, p <0.001).A cikin wannan binciken, mun bayar da rahoton m da ingantattun hanyoyin LC-MS/MS don kimanta magunguna da magunguna na MPA, waɗanda ke da isasshen kulawa don tantance ƙananan adadin lysates na PBMC da aka tattara jim kaɗan bayan HSCT.